马铃薯新品种‘吉薯1号’茎尖脱毒及组培快繁研究  

Research on Stem Tips Virus-free and Tissue Rapid Propagation Technology of New Potato Variety ’Jishu 1’

作　　者：李闯[1] 张海燕[1] 谭化[1] 王洋[1] 王凤[1] 张海[1] 王忠伟[1] LI Chuang;ZHANG Haiyan;TAN Hua;WANG Yang;WANG Feng;ZHANG Hai;WANG Zhongwei(Economic Plants Institute of Jilin Academy of Agricultural Sciences,Gongzhuling 136105,China)

机构地区：[1]吉林省农业科学院经济植物研究所,吉林公主岭136105

出　　处：《东北农业科学》2019年第6期62-64,73,共4页Journal of Northeast Agricultural Sciences

基　　金：吉林省农业委员会项目(3190104);吉林省农业科学院创新工程项目(C72083102)。

摘　　要：马铃薯茎尖脱毒技术是克服病毒侵害及解决马铃薯退化最为有效的途径。为保持马铃薯新品种‘吉薯1号’的品质,本研究以马铃薯新品种吉薯1号茎尖为外植体,对其脱毒方式和组织快繁培养基组分进行了优化。确定了以0.1%升汞8 min+75%酒精30 s对马铃薯茎尖脱毒后,用MS+6-BA 2 mg·L^-1+GA30.2 mg·L^-1+NAA 0.2 mg·L^-1培养基进行茎尖分化培养,再经MS+PIX 1.0 mg·L^-1培养基继代,可满足工厂化育苗需要。The technology of potato meristem detoxification is the most effective method to prevent the virus invasion and to solve the degradation of potato. To maintain the quality of the new potato variety ’Jishu 1’, the meristem of the new potato variety ’Jishu 1’ was were used as explants,the different disinfection methods and the tissue rapid propagation medium were optimized in this study. The potato mertem were first sterilized in 0.1% HgCl2 for 8 min and 75% alcohol for 30 s, and then MS+6-BA 2 mg·L^-1+GA30.2 mg·L^-1+NAA 0.2 mg·L^-1 medium were used for meristem induction,and MS+PIX 1.0 mg·L^-1 medium were used for subculture,these can meet the factory demand of reproduction.

关 键 词：马铃薯 吉薯1号 茎尖 脱毒 快繁 

分 类 号：S532[农业科学—作物学]