菊芋果聚糖合成酶基因1-FFT启动子克隆与功能分析  

Cloning and Fructan Synthase Gene 1-FFT Promoter in Jerusalem Artichoke(Helianthus tuberosus L.)

作　　者：孙雪梅 宗渊[1,2,5] 杨世鹏 王丽慧[4,5] 张怀刚 Sun Xuemei;Zong Yuan;Yang Shipeng;Wang Lihui;Zhang Huaigang(Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,810000;Qinghai Province Key Laboratory of Crop Molecular Breeding,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,810000;University of Chinese Academy of Sciences,Beijing,100049;Key Laboratory of vegetable Genetics and Physiology in Qinghai Province,Qinghai Academy of Agricultural and Forestry Sciences,Xining,810000;College of Agriculture and Animal Husbandry,Qinghai University,Xining,810000)

机构地区：[1]中国科学院西北高原生物研究所,西宁810000 [2]中国科学院西北高原生物研究所,青海省作物分子育种重点实验室,西宁810000 [3]中国科学院大学,北京100049 [4]青海省农林科学院,青海省蔬菜遗传与生理重点实验室,西宁810000 [5]青海大学农牧学院,西宁810000

出　　处：《分子植物育种》2020年第6期1792-1798,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31460523);青海省应用基础研究项目(2016-ZJ-751);青海省创新服务平台项目(2018-ZJ-T08);青海省创新平台建设专项(2017-ZJ-Y18)共同资助。

摘　　要：本研究通过Tail-PCR方法分离到菊芋1-FFT基因上游长度为1 611 bp的片段(FFTP)。神经网络启动子在FFTP序列中预测到8个启动子核心结构,瞬时表达结果显示起始密码子上游400 bp的启动子核心结构域与1 611 bp的启动GUS基因表达的活性无明显差异,表明-296到-246区域就是1-FFT基因的启动子区域。PlantCARE分析表明,FFTP序列中除基本启动子元件(TATA-box和CAAT-box)外,还含有与MYB转录因子结合的元件3个,参与光调控的顺式作用元件12个,茉莉酸反应、厌氧过程、脱落酸、抗逆反应的元件各2个,昼夜节律控制、生长素和赤霉素反应的元件各1个。这些顺式作用元件的鉴定为1-FFT参与相应的生物学过程提供理论依据。In this study,a 1611 bp fragment(FFTP),upsteam to 1-FFT,was isolated by Tail-PCR from Jerusalem artichoke.Neural network promoters predicted eight promoter core structures in the FFTP sequence.Because of there was no significant difference in the activity of GUS gene expression between the FFTP400 promoter core domain and FFTP promoter in transient expression,it could be inferred that the core promoter regions from-296 to-246 were the promoter regions of 1-FFT gene.PlantCARE analysis showed that besides the basic promoter elements(TATA-box and CAAT-box),FFTP sequence contained three elements binding to MYB transcription factor,12 cis-acting elements involved in light regulation,eight elements involved in jasmonic acid reaction,anaerobic process,abscisic acid and stress resistance,three elements relative to circadian rhythm control,auxin and gibberellin.The cis-acting elements identification provides a theoretical basis for 1-FFT to participate in the corresponding biological processes.

关 键 词：菊芋(Helianthus tuberosus L.) 果聚糖 1-FFT 启动子 功能分析 

分 类 号：S632.9[农业科学—蔬菜学]