喀斯特植物叶片草酸钙与抗坏血酸的相关性分析  

Correlation Analysis between Calcium Oxalate and Ascorbic Acid in Plant Leaf Grown in Karst Area

作　　者：苏志孟 张习敏[1,3] 刘伦衔 段盛光[3] 马琳 陈婷 乙引[1,2,3] Su Zhimeng;Zhang Ximin;Liu Lunxian;Duan Shengguang;Ma Lin;Chen Ting;Yi Yin(Key Laboratory of Plant Physiology and Development Regulation of Guizhou Province,Guizhou Normal University,Guiyang,550001;Key Laboratory of State Forestry Administration on Biodiversity Conservation in Karst Mountainous Areas of Southwestern China,Guizhou Normal University,Guiyang,550001;School of Life Science,Guizhou Normal University,Guiyang,550001)

机构地区：[1]贵州师范大学,贵州省植物生理与发育调控重点实验室,贵阳550001 [2]贵州师范大学,国家林业局西南喀斯特山地生物多样性保护重点实验室,贵阳550001 [3]贵州师范大学生命科学学院,贵阳550001

出　　处：《分子植物育种》2020年第6期2013-2022,共10页Molecular Plant Breeding

基　　金：国家自然科学基金委员会——贵州省人民政府喀斯特科学研究中心项目(U1812401);贵州省科技合作计划项目(黔科合LH字[2015]7777号)共同资助。

摘　　要：草酸钙晶体是一种典型的植物生物矿物,在植物的生长发育和抗逆性方面起着重要的作用。本研究以贵州喀斯特生境中适生草本、灌木和乔木植物为材料,通过光学显微成像观察草酸钙晶体的形态和分布特征。同时,分析抗坏血酸和草酸钙含量之间的相关性,探究抗坏血酸与草酸钙之间的关系。结果表明,植物叶片中草酸钙主要有簇晶和棱晶两种形态,簇晶主要分布在叶肉组织中,而棱晶主要分布在叶脉组织中。在草本和灌木物种中,草酸钙含量与抗坏血酸和脱氢抗坏血酸均表现为正相关关系;然而,在乔木物种中,草酸钙含量与抗坏血酸含量正相关,与脱氢抗坏血酸含量相关性不明显。研究发现,贵州喀斯特适生植物叶片主要以叶肉簇晶和叶脉棱晶的方式富集草酸钙晶体,植物抗坏血酸和草酸钙之间表现为正相关,这为进一步研究草酸钙晶体的合成和生物学功能提供了科学数据。Calcium oxalate crystal is a typical plant biomineral and plays an important role in plant growth and stress resistance.The morphology and distribution characteristics of calcium oxalate crystals were observed by microscopy in herbs,shrubs and arbor species growing in karst environment in Guizhou province.In addition,the correlation between calcium oxalate content and ascorbic acid(ASA)content and dehydrogenase ascorbic acid(DHA)content was also analyzed to reveal the relationship between ASA and calcium oxalate in this paper.The results showed that the calcium oxalate crystals in the leaves of plants had two shapes including cluster crystals and prismatic crystals in the tested species.The cluster crystals are mainly distributed in the mesophyll tissue,while the prismatic crystals are mainly distributed in the veins tissues.In herb and shrub species,calcium oxalate content was positively correlated with ASA and DHA;however,in arbor species,calcium oxalate content was positively correlated with ASA content but not correlated with DHA content.The study indicated that the leaves of species growing in the karst area were richment with calcium oxalate in mesophyll and vein tissue.ASA content and calcium oxalate content was positively correlated in Karst species.It provided scientific data for further study on the synthesis and biological functions of calcium oxalate crystals.

关 键 词：草酸钙 晶体形态 抗坏血酸 相关性分析 

分 类 号：Q945[生物学—植物学]