酚化改性木质素与纤维素酶相互作用研究  

Study on the Interaction Between Phenolized Lignin and Cellulase

作　　者：牟洪燕[1,2,3] 黄瑾 李维英 樊慧明[1,2] 詹怀宇[1] MOU Hongyan;HUANG Jin;LI Weiying;FAN Huiming;ZHAN Huaiyu(State Key Lab of Pulp and Paper Engineering,South China University of Technology,Guangzhou,Guangdong Province,510640;Experimental Teaching Demonstration Center of Light Industry and Food Science,South China University of Technology,Guangzhou,Guangdong Province,510640;Xinyichang Automation Equipment Co.,Ltd.,Shenzhen,Guangdong Province,518103)

机构地区：[1]华南理工大学制浆造纸工程国家重点实验室,广东广州510640 [2]华南理工大学轻工与食品实验教学示范中心,广东广州510640 [3]新益昌自动化设备有限公司,广东深圳518103

出　　处：《中国造纸》2020年第2期22-26,共5页China Pulp & Paper

基　　金：国家自然科学基金(31600469);广东省自然科学基金博士创业基金(2017A030332343)。

摘　　要：采用连苯三酚和间苯二酚对木质素进行酚化改性,使改性木质素的酚羟基含量增加,有利于纤维素酶的吸附应用。结果表明,经酚化改性后,木质素间苯二酚和木质素连苯三酚的最大吸附纤维素酶量分别为842.1 mg/g和911.4 mg/g。与原始酶活相比,木质素间苯二酚和木质素连苯三酚吸附的纤维素酶的酶活保留率分别为57%和38%。通过调节pH值为10,吸附的纤维素酶极易从木质素衍生物中分离。为全面解释纤维素酶吸附机理,对木质素衍生物的物化性质进行了表征。In this study,the lignin was modified by pyrogallol and resorcinol to increase the content of phenolic hydroxyl thereby in favour of cellulase adsorption application.After phenolation,the maximum adsorption cellulase capacity of lignoresorcinol and lignopyrogallol were 842.1 mg/g and 911.4 mg/g,respectively.Compared with original cellulase activity,the activity of the adsorbed cellulase on lignoresorcinol and lignopyrogallol still retained 57% and 38%.The adsorbed cellulase was easily separated from the lignin derivatives by adginting the pH value 10.Moreover,the physic-chemical properties of the lignin derivatives were characterized for explaining the cellulase adsorption mechanism.

关 键 词：酚化 木质素 纤维素酶 吸附 脱附 

分 类 号：TS7[轻工技术与工程—制浆造纸工程]