管花肉苁蓉药渣中多糖提取工艺及体外抗氧化活性研究  

Extraction Process of Polysaccharides from Cistanche tubulosa Residue and Their Antioxidant Activity In Vitro

作　　者：艾拉旦·麦麦提艾力 杨婷[1] 袁洁[2] 艾拉旦·艾力 姚军[1] 游林 MAIMAITIAILI Ailadan;YANG Ting;YUAN Jie;AILI Ailadan;YAO Jun;YOU Lin(School of Pharmacy,Xinjiang Medical University,Urumqi 830011,China;College of Houbo,Xinjiang Medical University,Klamayi 834000,China;Hotan Dichen Medicine Biotechnology Co.,Ltd.,Hotan 848000,China)

机构地区：[1]新疆医科大学药学院,新疆乌鲁木齐830011 [2]新疆医科大学厚博学院,新疆克拉玛依834000 [3]和田帝辰医药生物科技有限公司,新疆和田848000

出　　处：《化学与生物工程》2020年第2期36-41,共6页Chemistry & Bioengineering

基　　金：克拉玛依市创新人才工程项目(2019RC001 A-02)。

摘　　要：在单因素实验的基础上,通过正交实验优化了管花肉苁蓉药渣中多糖的提取工艺,并对其体外抗氧化活性进行了初步研究。结果表明,最佳提取工艺条件为:提取温度90℃、料液比1∶50(g∶mL)、提取时间2.0 h,在此条件下,管花肉苁蓉多糖提取率为5.76%;管花肉苁蓉多糖具有较高的抗氧化活性,其对DPPH自由基、羟基自由基具有清除能力,对铜离子有较强的还原能力。该提取方法简便易行,节能环保,适用于管花肉苁蓉多糖的提取。On the basis of single-factor experiments,we optimized the extraction process of polysaccharides from Cistanche tubulosa residue by orthogonal experiments,and preliminarily evaluated their antioxidant activity in vitro.The results show that the extraction rate of polysaccharides from Cistanche tubulosa is 5.76%under the optimal extraction conditions as follows:the extraction temperature of 90℃,the solid-liquid ratio of 1∶50(g∶mL),and the extraction time of 2.0 h.The polysaccharides from Cistanche tubulosa have high antioxidant activity,have scavenging ability to DPPH free radicals and hydroxyl free radicals,and have strong ability in reducing copper ions.The extraction method is simple,feasible,energy saving,and environmentally friendly,which is suitable for the extraction of polysaccharides from Cistanche tubulosa.

关 键 词：管花肉苁蓉 多糖 提取工艺 正交实验 体外抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]