猪德尔塔冠状病毒变异株CHN-GX01-2018株全基因序列分析  

Genomic characteristics of the complete gene of PDCoV CHN-GX-2018strain

作　　者：张世亨 黄海鑫 尹彦文 施开创 郑敏 汪伟 曹亮[2,4] 孙文超 鲁会军[2,4] 金宁一[2,4] ZHANG Shi-heng;HUANG Hai-xin;YIN Yan-wen;SHI Kai-chuang;ZHNEG Min;WANG Wei;CAO Liang;SUN Wen-chao;LU Hui-jun;JIN Ning-yi(Institute of Virology,Wenzhou University,Wenzhou,Zhejiang 325035,China;College of Animal Science and Technology,Jinlin Agricultural University,Changchun 130118,China;Guangxi Center for Animal Disease Control and Prevention,Nanning 530001,China;Military Veterinary Institute,Academy of Military Medical Sciences,Changchun 130122,China)

机构地区：[1]温州大学病毒学研究所,浙江温州325035 [2]吉林农业大学动物科学技术学院,吉林长春130118 [3]广西壮族自治区动物疫病预防控制中心,广西南宁530001 [4]军事医学研究院军事兽医研究所,吉林长春130122

出　　处：《中国兽医学报》2020年第3期483-490,共8页Chinese Journal of Veterinary Science

基　　金：国家重点研发计划资助项目(2018YFD0500104);温州市基础性科研项目(N20180010,N20190005);广西科技重大专项(桂科AA17204057);广西水产畜牧科技项目(桂渔牧科201528017)。

摘　　要：为了解广西PDCoV流行毒株在猪群中的感染和流行特点,用设计的25对特异性引物从腹泻样本中检测获得1株PDCoV全基因组序列。结果显示,CHN-GX01-2018株基因组全长25406bp,与美国毒株比较存在3处基因缺失,分别位于ORF1a/1b第400~403aa、第756~759aa位置及S基因存在1个氨基酸的缺失。全基因组与S基因遗传进化分析结果显示,CHN-GX01-2018与代表株CHN-AH-2004(安徽株)同源性为97.4%。然而,同源性分析显示中国PDCoV株也存在不同的进化分支,表明PDCoV在中国猪群中不断进化。Porcine deltacoronavirus(PDCoV)is a newly emerging enterotropic swine coronavirus that causes acute enteritis in nursing piglets.In order to understand the infection and epidemic characteristics of Guangxi PDCoV strains in pigs,25pairs of specific primers were designed to detect the full length of PDCoV genome from diarrhea samples.The results showed that the genome of CHN-GX01-2018strain is 25,406bp in length,and there are three gene deletions compared with the US strain:at 400-403aa,756-759aa in located at ORF1a/1b,and the Sgene has one amino acid deletion.Homology analysis showed that genetic analysis of whole genome and Sgene showed that CHN-GX01-2018had 97.4%similarity with CHN-AH-2004(Anhui strain).However,there are also different evolutionary branches of the Chinese PDCoV strain,indicating that PDCoV is constantly evolving in Chinese pig herds.This study plays a positive role in the epidemiological status of PDCoV in the herd and its genetic evolution,vaccine development,and disease prevention and control.

关 键 词：猪德尔塔冠状病毒 全基因组序列 遗传变异 

分 类 号：S852.65[农业科学—基础兽医学]