家兔前体脂肪细胞分化不同时期基因表达谱分析  

Analysis of gene expression profiles at different stages during preadipocyte differentiation in rabbits

作　　者：杜坤 毛初阳 任安勇 吴雪梅 李庆玲 陈婷婷 陈仕毅 赖松家 Kun Du;Chuyang Mao;Anyong Ren;Xuemei Wu;Qingling Li;Tingting Chen;Shiyi Chen;Songjia Lai(Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province,Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]四川农业大学,畜禽遗传资源发掘与创新利用四川省重点实验室,成都611130

出　　处：《遗传》2020年第3期309-320,I0010,共13页Hereditas（Beijing)

基　　金：国家现代农业产业技术体系建设项目(编号:CARS-44-A-2)资助。

摘　　要：脂肪的过度积累严重危害人类健康。前体脂肪细胞分化是脂肪发育的关键过程,研究前体脂肪细胞分化相关基因的表达有助于认识脂肪沉积的机理。尽管家兔是一种理想的研究脂肪发育的动物模型,但是针对其前体脂肪细胞分化不同时期基因表达谱的研究鲜见报道。本研究通过诱导家兔前体脂肪细胞分化,在分化第0 d、3 d和9 d收集脂肪细胞,利用转录组测序(RNA-seq),在分化第3 d样本与第0 d样本的比较中筛选出1352个差异表达基因(differentially expressed genes, DEGs),在分化第9 d样本与第3 d样本的比较中筛选出888个DEGs。GO (gene ontology)功能富集和KEGG (kyoto encyclopedia of genes and genomes)通路分析发现,0~3 d分化期上调的DEGs显著富集在PPAR信号通路和PI3K-Akt信号通路上,3~9d分化期上调的DEGs显著富集到与细胞周期调控有关的GO条目和KEGG信号通路,0~3d和3~9d阶段特异上调的DEGs可能分别作用于细胞质和细胞核。通过DEGs的蛋白-蛋白互作(protein-protein interaction, PPI)网络分析发现,筛选出的核心节点(hub node)基因可能通过调控细胞周期而影响家兔前体脂肪细胞分化。Excessive accumulation of fat is harmful to human health. The preadipocyte differentiation is a critical process of fat development. Studying the expression profiles of genes related to preadipocyte differentiation contributes to understanding of the mechanism of fat accumulation. Despite being considered an ideal animal model for studying adipogenesis, little is known about the gene expression profiles at different stages during preadipocyte differentiation in rabbits. In the present study, rabbit preadipocytes were cultured in vitro and induced for differentiation, and gene expression profiles of adipocytes collected at days 0, 3, and 9 of differentiation were analyzed by RNA-seq. We identified 1352 differentially expressed genes(DEGs) when comparing day 3 with day 0 and identified 888 DEGs when comparing day 9 with day 3. Gene Ontology(GO) enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis showed that the PPAR signaling pathway and PI3K-Akt signaling pathway were significantly enriched by the DEGs that up-regulated within the period of day 0 – day 3, and the GO terms and KEGG pathways that were associated with cell cycle were enriched by the DEGs that up-regulated within the period of day 3 – day 9. The DEGs that specifically up-regulated within the period of day 0 – day 3 might play roles in the cytoplasm, and the DEGs that specifically up-regulated within the period of day 3 – day 9 might act in the nucleus. The protein-protein interaction(PPI) network constructed by DEGs showed that hub node genes might modulate rabbit preadipocyte differentiation via regulating cell cycle.

关 键 词：脂肪细胞 分化 家兔 前体脂肪细胞 RNA-SEQ 

分 类 号：R589.2[医药卫生—内分泌] R-332[医药卫生—内科学]