半乳糖凝集素3表达抑制对人胃癌MGC-803细胞中Bcl-2和Bax表达的影响及其促凋亡作用  

Effect of galectin-3 expression suppression on expressions of Bcl-2 and Bax in gastric cancer MGC-803 cells and its promotion on apoptosis

作　　者：吴雪艳[1] 赵依纳 王小杰[3] 麻雯熠 王新杰[5] 李欣[4] WU Xueyan;ZHAO Yina;WANG Xiaojie;MA Wenyi;WANG Xinjie;LI xin(Department of Human Anatomy,Chengde Medical College,Chengde 067000,China;Department of Reproductive Medicine,Hebei Maternity Hospital,Shijiazhuang 050000,China;Institute of Basic Medicine,Chengde Medical College,Chengde 067000,China;Department of Histology and Embryology,Chengde Medical College,Chengde 067000,China;Department of Oncology,Chengde Central Hospital,Hebei Province,Chengde 067000,China)

机构地区：[1]承德医学院人体解剖学教研室,河北承德067000 [2]河北生殖妇产医院生殖医学科,河北石家庄050000 [3]承德医学院基础医学研究所,河北承德067000 [4]承德医学院组织与胚胎学教研室,河北承德067000 [5]河北省承德市中心医院肿瘤内科,河北承德067000

出　　处：《吉林大学学报（医学版）》2020年第2期335-339,共5页Journal of Jilin University：Medicine Edition

基　　金：河北省教育厅科研基金资助课题(ZD2018058);河北省卫健委科研基金资助课题(20181146);河北省高校重点发展学科建设项目资助课题(201304);承德医学院科研基金资助课题(201820)。

摘　　要：目的:探讨抑制半乳糖凝集素3(galectin-3)的表达对人胃癌MGC-803细胞凋亡的影响,为基因靶向治疗提供潜在靶点。方法:人胃癌MGC-803细胞分为siRNA干扰组、空白对照组和阴性对照组,siRNA干扰组MGC-803细胞转染靶向galectin-3的siRNA,空白对照组MGC-803细胞只加转染试剂,阴性对照组MGC-803细胞转染阴性对照的siRNA。采用流式细胞术和碘化丙啶(PI)染色法检测各组细胞凋亡率和细胞凋亡情况,Western blotting法检测各组细胞中galectin-3、Bcl-2和Bax蛋白表达情况。结果:与阴性对照组和空白对照组比较,siRNA干扰组细胞中galectin-3蛋白表达水平明显降低(P<0.05)。PI染色,siRNA干扰组凋亡细胞数量明显增加,凋亡细胞出现细胞核固缩、染色质浓集、新月形和凋亡小体等细胞凋亡特征,阴性对照组仅有少量散在的凋亡细胞。流式细胞术和Western blotting法检测,与阴性对照组和空白对照组比较,siRNA干扰组细胞凋亡率明显升高(P<0.05),Bcl-2蛋白表达水平明显降低(P<0.05),Bax蛋白表达水平未发生明显变化(P>0.05)。结论:抑制galectin-3表达可促进MGC-803细胞的凋亡,提示galectin-3对胃癌细胞发挥癌基因作用,具有成为靶向治疗靶点的可能性。Objective:To investigate the influence of galectin-3 expression suppression in the apoptosis of human gastric cancer MGC-803 cells,and to provide a potential target for gene targeting therapy.Methods:The human gastric cancer MGC-803 cells were selected and divided into siRNA interference group,blank control group and negative control group.The MGC-803 cells in siRNA interference group were transfected with the siRNA which targeted galectin-3,the MGC-803 cells in blank control group were added only transfection reagent,while the MGC-803 cells in negative control group were transfected with negative siRNA.Flow cytometry and propidium iodide(PI)staining method were used to detect the apoptosis.Western blotting method was applied to determine the expressions of galectin-3,Bcl-2 and Bax proteins.Results:Compared with negative control group and blank control group,the expression level of galectin-3 protein in siRNA interference group was significantly decreased(P<0.05).The PI staining results showed that the number of apoptotic cells in siRNA interference group was increased significantly in which nuclear pyknosis,chromatin concentration,crescent shape and apoptotic bodies were found;meanwhile only a small amount of apoptotic cells in negative control group.The flow cytometry and Western blotting results showed that compared with negative control group and blank control group,the apoptotic rate of the MGC-803 cells in siRNA interference group was significantly increased(P<0.05),the expression level of Bcl-2 protein was decreased significantly(P<0.05),and the expression level of Bax protein had no significant difference(P>0.05).Conclusion:To suppress galectin-3 expression can promote the apoptosis of MGC-803 cells,suggesting that galectin-3 might play an oncogene role in the gastric cancer cells and has the possibility of becoming a target for targeting therapy.

关 键 词：半乳糖凝集素3 MGC-803细胞 Bcl-2 BAX 流式细胞术 

分 类 号：R735.2[医药卫生—肿瘤]