抗革兰氏阴性菌耐格霉素的形式合成  

Formal Synthesis of Gram-Negative Antibiotic Negamycin

作　　者：张世举 李晓彤[2] 王燕 郑宇璁 韩世清[2] 郁惠蕾[4] 黄莎华 Zhang Shiju;Li Xiaotong;Wang Yan;Zheng Yucong;Han Shiqing;Yu Huilei;Huang Shahua(School of Chemical and Environmental Engineering,Shanghai Institute of Technology,Shanghai 201418;College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211816;Key Laboratory of Synthetic Chemistry of Natural Substances,Center for Excellence in Molecular Synthesis,Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences,Shanghai 200032;State Key Laboratory of Bioreactor Engineering,Shanghai Collaborative Innovation,Center for Biomanufacturing,East China University of Science and Technology,Shanghai 200237)

机构地区：[1]上海应用技术大学化学与环境工程学院,上海201418 [2]南京工业大学生物与制药工程学院,南京211816 [3]中国科学院上海有机化学研究所分子合成卓越中心天然产物有机合成化学重点实验室,上海200032 [4]华东理工大学生物反应器工程国家重点实验室上海生物制造技术协同创新中心,上海200237

出　　处：《有机化学》2020年第2期521-527,共7页Chinese Journal of Organic Chemistry

基　　金：国家自然科学基金(No.21402121);上海市杨帆计划(No.16YF1414400)资助项目.

摘　　要：耐格霉素是具有抗革兰氏阴性菌活性的天然产物.以廉价易得的3-羰基-4-氯丁酸乙酯为原料,以八步29%的总收率实现了耐格霉素的形式合成.该工作改进了文献的合成路线,利用生物催化不对称还原高立体选择性引入C-5位的手性羟基,并将危险的叠氮引入反应放在合成后期,降低合成路线的操作风险.分子中C-3位的仲碳胺基手性中心通过Ellamn试剂介导的不对称Mannich反应构建.该路线易于放大,有望为构建耐格霉素类似物分子库以及高通量药物筛选奠定基础.Negamycin is a potent gram-negative antibiotic.By using commercial available ethyl 4-chlorobutyrate as starting material,the formal synthesis of negamycin was achieved within 8 steps and 29%overall yield.This modified synthetic route features in-situ enzymatic promoted asmmetric reduction reaction to introduce chiral hydroxy group at C-5,a late-stage azidination at C-6 to avoid the introduction of explosive azide group in the early stage in previous syntheses.The C-3 aza-chiral center was constructed via Ellman reagent-based asymmetric Mannich reaction.This efficient route is scalable and suitable to establish a library of negamycin analogues for future high-throughput screening.

关 键 词：耐格霉素 MANNICH反应 革兰氏阴性菌 形式合成 

分 类 号：TQ465[化学工程—制药化工]