人羊水干细胞移植对大鼠四氯化碳诱导性肝硬化的治疗作用及其机制  

Effect of Human Amniotic Fluid Stem Cell Transplantation on Carbon Tetrachloride Induced Cirrhosis in Rats and its Mechanism

作　　者：李盛[1] 焦志勇[1] 饶佩斯 钟燕明 LI Sheng;JIAO Zhi-yong;RAO Pei-si(Department of Gastroenterology,Yuebei People's Hospital,Shaoguan 512026,China)

机构地区：[1]粤北人民医院消化内科,广东韶关512026

出　　处：《医学临床研究》2020年第3期329-332,335,共5页Journal of Clinical Research

基　　金：广东省科技发展专项资金(编号:2016A030313016)。

摘　　要：【目的】探讨人羊水干细胞移植对大鼠四氯化碳诱导性肝硬化的治疗作用及其机制。【方法】采用60%的四氣化碳植物油皮下注射7周制造肝硬化大鼠模型,再随机分成对照组(注射等体积PBS)、hAFSC移植组和hAFSC移植后基质细胞衍生因子Ⅰ(SDF-1)受体CXCR4的特异性阻断剂普乐沙福(AMD3100)干预组,处理3周后处死所有大鼠,检测大鼠血清碱性磷酸酶(ALP)、白蛋白(ALB)和胆固醇(Ch),按Ishak评分标准评估肝硬化情况。体外实验分为3个组:对照组(等剂量PBS)、AMD3100组、AMD3100+SDF-1组,观察不同处理因素对hAFSC的存活、迁移、黏附的影响。【结果】与对照组比较,移植组的血清ALB和Ch均显著升高(P<0.05),ALP无显著变化(P>0.05),而干预组的ALP、ALB及Ch与对照组比较差异均无统计学意义(P>0.05)。移植组与对照组肝组织纤维化评分比较差异有统计学意义(P=0.005),移植组与干预组也有显著差异(P=0.022)。体外实验结果表明:SDF-1能缓解AMD3100对hAFSC的迁移、存活、黏附的抑制(P<0.05)。【结论】hAFSC经静脉肝内移植可减轻大鼠四氯化碳诱导性肝硬化病变程度,其机制与SDF-1/CXCR4调控有关。【Objective】To investigate the therapeutic effect and mechanism of human amniotic nuid stem cell transplantation on carbon tetrachloride induced cirrhosis in rats.【Methods】Cirrhotic rats were made by injecting 60% carbon tetrachloride vegetable oil subcutaneously for 7 weeks,and then randomly divided into control group(injecting PBS of equal volume),,hAFSC transplantation group and the intervention group of praxafu(AMD3100),a specific blocker of CXCR4 receptor of stromal cell-derived factor-1(SDF-1)after the transplantation of AFSC.After 3 weeks of treatm ent,all rats were killed,serum A LP,ALB and CH were measured,and cirrhosis was evaluated according to Ishak scoring standard.In vitro experiments were divided into three groups:control group(PBS),AMD3100 group and AMD3100+SDF-1 group.The effects of different treatment factors on the survival,migration and adhesion of HFSC were observed.【Results】Compared with the control group,the serum ALB and Ch in the transplantation group were significantly higher(P<0.05),and ALP had no significant change(P>0.05),while ALP,ALB and Ch in the intervention group were not significantly different from those in the control group(P>0.05).Liver tissue fibrosis scores were statistically significant between the transplant and control groups(P=0.005),and significant differences between the transplant and the intervention groups(P=0.022).The results The results of in vitro experiments showed that SDF-1 could alleviate the inhibition of AMD3100 on the migration,survival and adhesion of hAFSC(P<0.05).【Conclusion】hAFSC transvenous intrahepatic transplantation reduces the extent of carbon tetrachloride-induced cirrhosis in rats and its mechanism is related to SDF-1/CXCR4 regulation.

关 键 词：肝硬化 实验性 大鼠 四氯化碳 疾病模型 动物 

分 类 号：R575.2[医药卫生—消化系统]