低强度恒定磁场对肿瘤细胞铁死亡的作用  

Effect of low intensity constant magnetic field on tumor ferroptosis

作　　者：胡金翠 许建文[1] 胡榜利[1] 朱庭沛 黄潇潇 HU Jincui;XU Jianwen;HU Bangli;ZHU Tingpei;HUANG Xiaoxiao(Department of Rehabilitation,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学第一附属医院康复医学科,南宁市530021

出　　处：《微创医学》2020年第1期2-4,45,共4页Journal of Minimally Invasive Medicine

基　　金：广西壮族自治区临床重点专科建设项目(编号:桂卫医发[2018]6号);国家自然科学基金项目(编号:81860417);研究生教育创新计划项目(编号:YCSW2020118)。

摘　　要：目的研究低强度恒定磁场对结肠癌细胞铁死亡的影响。方法用结肠癌HCT116细胞建立细胞模型,分为空白对照组、Erastin组、Erastin+磁场组及抑制剂组。干预48 h后,对比分析4组的铁含量、脂质过氧化物-丙二醛(MDA)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GPX)等铁死亡指标以及细胞迁移情况。结果培养48 h后,与空白对照组相比,Erastin组、Erastin+磁场组的铁含量,Erastin组、Erastin+磁场组、抑制剂组的MDA含量均增高;且Erastin+磁场组高于Erastin组(均P<0.05)。与空白对照组相比,Erastin组、Erastin+磁场组、抑制剂组的GSH含量和GPX活性,Erastin组、Erastin+磁场组的划痕修复率均降低,且Erastin+磁场组低于Erastin组(均P<0.05)。结论低强度恒定磁场有增强肿瘤细胞铁死亡的作用,但其作用机制还需进一步研究。Objective To study the effect of low intensity constant magnetic field on ferroptosis of colon cancer.Methods Colon cancer HCT116 cells were used to establish cell model,which were divided into blank control group,Erastin group,Erastin+magnetic field group and inhibitor group.After 48 hours of intervention,the ferroptosis indicators,including iron content,lipid peroxide-malondialdehyde(MDA),glutathione(GSH)and glutathione peroxidase(GPX),as well as cell migration of the four groups were compared and analyzed.Results After 48 hours of culture,in comparison with the blank control group,iron contents in the Erastin and Erastin+magnetic field groups,and MDA contents in the Erastin,Erastin+magnetic field and inhibitor groups all increased,with the contents higher in the Erastin+magnetic field group than in the Erastin group(all P<0.05).Compared to the blank control group,the GSH contents and GPX activities of the Erastin,Erastin+magnetic field and inhibitor groups,and the scratch repair rate in the Erastin and Erastin+magnetic field groups all decreased,with the indicators mentioned above lower in the Erastin+magnetic field group than in the Erastin group(all P<0.05).Conclusion Low intensity constant magnetic field can enhance the effect of tumor ferroptosis,but its mechanism still needs further research.

关 键 词：磁场 铁死亡 Erastin 肿瘤康复 

分 类 号：R73-351[医药卫生—肿瘤]