基于灰色关联度法分析刺五加种子中胚率、内源激素和酶活力的相关性  

Grey Correlation Analysis on Rate of Embryo,Endogenous Hormone and Enzyme Activity of Acanthopanax senticosus Seeds

作　　者：付士朋 沈宏伟 王谦博[3] 李俊萍 王聪[1] 张爽[1] 刘悦 郭盛磊[1,4] 王振月 FU Shi-peng;SHEN Hong-wei;WANG Qian-bo;LI Jun-ping;WANG Cong;ZHANG Shuang;LIU Yue;GUO Sheng-lei;WANG Zhen-yue(Heilongjiang University of Chinese Medicine,Harbin 150040,China;Jiangyin Tianjiang Pharmaceutical Co.Ltd.,Harbin 150040,China;The First Affiliated Hospital,School of Clinic Medicine of Guangdong Pharmaceutical University,Guangzhou 510000,China;Postdoctoral Programme of Heilongjiang Zbd Pharmaceutical Co.Ltd.,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学,哈尔滨150040 [2]江阴天江药业有限公司,江苏江阴214400 [3]广东药科大学附属第一医院,广州510000 [4]黑龙江珍宝岛药业博士后科研工作站,哈尔滨150040

出　　处：《中国实验方剂学杂志》2020年第4期145-151,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点研发计划项目(2016YFC0500303);国家科技重大专项和重点研发项目省级项目(GX17C006);黑龙江省博士后项目(LBH-Z17208)。

摘　　要：目的:研究刺五加种子在层积过程中的内源激素含量、相关酶活力与胚率的相关性研究,为生产和科研中应用内源激素和相关生理指标来调控刺五加种子萌发提供理论依据。方法:采用刺五加内生真菌,不同浓度的一氧化氮和过氧化氢溶液进行浸种处理后对刺五加种子进行变温层积处理,利用高效液相色谱法测定刺五加种子中内源激素[赤霉素(GA3),脱落酸(ABA),吲哚乙酸(IAA),吲哚丁酸(IBA)及水杨酸(SA)]的含量,并且检测体内酶[过氧化氢酶(CAT),超氧化物歧化酶(SOD),过氧化物酶(POD),丙二醛(MDA)]活力变化。通过灰色关联度法探讨胚率和刺五加种子中激素和其酶活力的相关性。结果:胚率与IAA,GA3,CAT有显著正相关联,关联系数分别为1.086,0.935,1.067,IAA,GA3,CAT,IBA,SA之间存在显著的正相关性,与ABA,MDA,POD之间具有明显的负相关性,SOD的活性与其他含量之间无显著的相关性。关联度大小比较为IAA>CAT>GA3>IBA>SA>SOD>ABA>POD>MDA。结论:IAA,GA3,CAT对胚率有明显促进作用,激素含量与酶活力之间有明显的相关性,可为探讨刺五加种子萌发机制提供理论依据。Objective:To study the correlation between endogenous hormone content,related enzyme activity and embryogenesis during the stratification of Acanthopanax senticosus seeds,and to provide theoretical basis for application of endogenous hormones and related physiological indicators in regulating germination of A.senticosus seeds in production and scientific research.Method:Endophytic fungi as well as different concentrations of nitric oxide and hydrogen peroxide solution were used for soaking the seeds of A.senticosus,and then thermophilic stratification was conducted for the seeds.The content of endogenous hormones such as gibberellin(GA3),abscisic acid(ABA),indole acetic acid(IAA),indole butyric acid(IBA)and salicylic acid(SA)in seeds of A.senticosus were tested by high performance liquid chromatography(HPLC),and the activity change of enzymes in vivo such as catalase(CAT),superoxide dismutase(SOD),peroxidase(POD),and malondialdehyde(MDA)were tested.The correlation between the embryo rate and the hormones and their enzyme activities in the seeds of A.senticosus was studied by the grey correlation method.Result:The embryo rate was significantly positively correlated with IAA,GA3,and CAT,with correlation coefficient of 1.086,0.935 and1.067,respectively.There was a significant positive correlation between IAA,GA3,CAT,IBA,and SA,but with a significant negative correlation with ABA,MDA,and POD.The correlation degree was as follows:IAA>CAT>GA3>IBA>SA>SOD>ABA>POD>MDA.Conclusion:IAA,GA3 and CAT have significant promoting effects on embryo rate,and there is a significant correlation between hormone content and enzyme activity,which provides a basis for exploring the seed germination mechanism of A.senticosus.

关 键 词：刺五加种子 激素含量 酶活力 灰色关联度分析 相关性分析 

分 类 号：R284.2[医药卫生—中药学] R289[医药卫生—中医学]