便携式405 nm激光诱导荧光检测仪的研制和性能评估  

Development and Performance Evaluation of Portable 405 nm Laser-induced Fluorescence Detector

作　　者：张玲玲[1,2] 陈媛 王彩霞[1,3] 王春霞 李国旗 孙杰 王健超[1] 贾锋 Zhang Lingling;Chen Yuan;Wang Caixia;Wang chunxia;Li Guoqi;Sun Jie;Wang Jianchao;Jia Feng(Shanghai Institute of Laser Technology,Shanghai 200233,China;Shanghai Laser DMP traceability Engineering Research Center,Shanghai 200233,China;Shanghai Engineering Research Center of LaserMedical Equipmen,Shanghai 200233,Shanghai 200233,China;Shanghai General Recognition Technology institute,Shanghai 200231,China;Shanghai Instrument Research Institute,Shanghai 200063,China)

机构地区：[1]上海市激光技术研究所,上海200233 [2]上海激光直接物标溯源工程技术研究中心,上海200233 [3]上海医学设备工程技术研究中心,上海200233 [4]上海通用识别技术研究所,上海200231 [5]上海仪器仪表研究所,上海200063

出　　处：《应用激光》2019年第6期1035-1040,共6页Applied Laser

基　　金：上海市科委资助项目(项目编号:19441911700,16142200400)。

摘　　要：研制了一款模块化便携式的激光诱导荧光的检测仪。本检测仪以光纤耦合的稳谱405 nm LD激光器作为激发光源,以改进的共聚焦光学结构的荧光探头作为荧光的收集模块,微型光栅光谱仪作为荧光光谱分析模块。该检测仪采用模块化结构,具有体积小、成本低、灵敏度高、易操作等特点。以荧光素钠为测试样品,对仪器进行了评估。水的拉曼S/N值为935.67,检测仪的最低检出限为1.9×10^-11 g/L;溶液浓度在10^-10到10^-12 g/L的范围内,荧光强度的线性相关系数达到0.9998,线性范围超过3个数量级;重复性测试中,荧光峰值强度的相对标准偏差为RSD=3.36%,荧光波长相对标准偏差RSD=0.14%。结果表明该检测仪具有灵敏度高、重复性好、线性范围宽等特点,满足现场物品的实时快速检测需求。A portable laser-induced fluorescence detector based on module design,which includes an excitation source of 405 nm fibre-coupled stable spectra diode laser,a fluorescence collection module based on a fibre fluorescence optical f probe with a improved confocal optical path,a fluorescence analysis module of Mini Fiber Grating Spectrometer.The advantages of the detector is compact,small size,low cost,high sensitivity,easy to operate.The performance of the detector was evaluated by Fluorescein Sodium.Water raman peak measurement S/N is 935.67.The detection limit of Fluorescein Sodium was 1.9×10^-11 g/L.Correlation of the fluorescence intensity was 0.9998 with the concentration from 10^-10 to 10^-12 g/L,the linear dynamic range was over 3 order.RSD of the fluorescence wavelength and intensity reproducibility was 0.14%and 3.36%respectively.It was concluded that the 405 nm LIFD had highly sensitivity,good reproducibility,a wide linear range.

关 键 词：LD激光器 激光诱导荧光 荧光检测 

分 类 号：O433.5[机械工程—光学工程]