雌激素作用下输卵管上皮细胞S100A8和S100A9的表达及其作用探讨  

Expression of S100A8 and S100A9 in oviduct epithelial cells induced by estrogen and their possible role in oviduct

作　　者：李晓丹 曹贵方[1] 杨宏新 于建 刘默宁 龙鑫 魏巍 李春芳 苏红[1] LI Xiao-dan;CAO Gui-fang;YANG Hong-xin;YU Jian;LIU Mo-ning;LONG Xin;WEI Wei;LI Chun-fang;SU Hong(Inner Mongolia Agricultural University, Inner Mongolia Key Lab of Basic Veterinary Science, Hohhot 010018, China;Dept of Cell Biology, College of Preclinical Medicine, Inner Mongolia Medical University, Hohhot 010110, China)

机构地区：[1]内蒙古农业大学内蒙古自治区基础兽医学重点实验室,内蒙古自治区呼和浩特010018 [2]内蒙古医科大学基础医学院,细胞生物学教研室,内蒙古自治区呼和浩特010110

出　　处：《中国药理学通报》2020年第4期562-568,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 31360593);内蒙古医科大学青年基金资助项目(No YKD2015QNCX002);内蒙古医科大学实验室开放基金资助项目(No 2019ZN32)。

摘　　要：目的研究基因S100A8和S100A9是否可能参与雌激素对输卵管的功能调控,并探讨它们对输卵管可能的影响。方法免疫组织化学法检测S100A8和S100A9在健康间情期,绵羊输卵管壶腹部中的基础表达及分布;利用E2作用绵羊输卵管上皮细胞,在不同作用时间及不同浓度E2作用下,q-PCR及免疫荧光法检测输卵管上皮细胞S100A8和S100A9 mRNA及蛋白的表达情况。结果在健康间情期绵羊输卵管,S100A8和S100A9高表达于黏膜上皮及腺上皮,并在血管中也有表达。输卵管上皮细胞在高浓度E2作用下,S100A8和S100A9的表达在不同时间出现动态变化,并均在E2作用6 h后出现表达高峰,在该时间下不同浓度的E2均显著诱导S100A8和S100A9的高表达,但在10-7 mol·L^-1和10-8 mol·L^-1浓度下表达最高,两组差异不显著。结论输卵管上皮细胞中S100A8和S100A9受到雌激素调控,高浓度雌激素促进S100A8和S100A9的高表达,可能与交配时生殖道的天然防御有关,并可能参与卵子在输卵管的运输。Aim To study whether the genes S100 A8 and S100 A9 are related to the functional regulation of oviduct by estrogen, and to explore their possible effects on fallopian tubes. Methods The basic expression and distribution of S100 A8 and S100 A9 in ampullary oviduct tissues of healthy sheep in diestrum were verified by immunohistochemistry. The expressions of S100 A8 and S100 A9(mRNA and protein) in oviduct epithelial cells were detected by q-PCR and immunofluorescence, the cells were treated by E2 at different time points and different concentrations. Results S100 A8 and S100 A9 were highly expressed in mucosal epithelium and glandular epithelium of sheep uterine tubes during the diestrum period, and in blood vessels as well. The expression of S100 A8 and S100 A9 in tubal epithelial cells changed dynamically at different time ponits under the action of high concentration of E2 in vitro, and reached the peak 6 hours after E2 treatment. At this time, different concentrations of E2 significantly induced the high expression of S100 A8 and S100 A9, but the expressions of S100 A8 and S100 A9 were the highest at the concentrations of 10-7 mol·L-1 and 10-8 mol·L-1, and there was no significant difference between the two groups. Conclusions S100 A8 and S100 A9 in oviduct epithelial cells are regulated by estrogen. Under the regulation of high concentration estrogen, the high expression of S100 A8 and S100 A9 may be related to the natural defense of reproductive tract during mating, and may be involved in the transport of eggs in fallopian tube.

关 键 词：雌激素 输卵管 S100A8 S100A9 天然防御 卵子运输 

分 类 号：R-332[医药卫生] R322.65