七叶皂苷钠通过抑制4EBP1的磷酸化进而减缓CCl4诱导的大鼠肝纤维化  

Sodium aescinate alleviates CCl4-induced liver fibrosis in rats by inhibiting phosphorylation of 4EBP1

作　　者：李扬 周大臣[1] 谢海深 刘付宝[2] 熊奇如 耿小平[1] LI Yang;ZHOU Da-chen;XIE Hai-shen;LIU Fu-bao;XIONG Qi-ru;GENG Xiao-ping(Dept of Hepatobiliary and Pancreatic Surgery,the Second Hospital of Anhui Medical University,Hefei 230601,China;Dept of Hepatobiliary and Pancreatic Surgery,the First Affiliated Hospital of Anhui Medical University Gao-Xin Branch,Hefei 230022,China)

机构地区：[1]安徽医科大学第二附属医院肝胆胰外科,安徽合肥230601 [2]安徽医科大学第一附属医院高新院区肝胆胰外科,安徽合肥230022

出　　处：《中国药理学通报》2020年第4期577-582,共6页Chinese Pharmacological Bulletin

基　　金：安徽省科技厅重点研究与开发计划项目(No 1804h08020239)。

摘　　要：目的研究七叶皂苷钠(Sodium Aescinate,SA)对四氯化碳诱导的大鼠肝纤维化的抑制作用。方法41只健康♂SD大鼠随机分为正常对照组(n=5)、模型组(n=18)以及七叶皂苷钠治疗组(n=18)。在8周实验结束后取大鼠肝脏组织,HE染色观察肝脏组织的形态结构,Masson染色观察胶原纤维增生,免疫组化检测相关蛋白的表达;MTT法测定大鼠肝星状细胞HSC-T6细胞增殖,流式细胞仪检测细胞凋亡,Western blot法检测细胞蛋白的表达。结果七叶皂苷钠可以抑制四氯化碳诱导的大鼠肝纤维化形成,抑制肝星状细胞的增殖,并能促进其凋亡,且能抑制p-4EBP1、I型胶原及Ⅲ型胶原的表达。结论七叶皂苷钠通过抑制4EBP1磷酸化进而抑制肝脏纤维化的进程。Aim To study the inhibitory effects of sodium aescinate in liver fibrosis induced by carbon tetrachloride(CCl4).Methods Forty-one male SD rats were recruited in this study and randomized into control group(n=5),model group(n=18),and sodium aescinate treated group(n=18).Masson staining was performed for collagen fiber detecting,and IHC staining was conducted for accessing the expression of interest proteins in rat liver.MTT and apoptosis assay were performed to evaluate the effects of sodium aescinate on HSC-T6 cells.The expression of interest proteins was detected by immunoblotting.Results Sodium aescinate alleviated the liver fibrosis induced by CCl4 through proliferation inhibition and apoptosis induction in HSC-T6 cells.Sodium aescinate also down-regulated the phosphorylation of 4EBP1,the expression of collagen I and collagenⅢ.Conclusion Sodium aescinate alleviates liver fibrosis induced by CCl4.

关 键 词：七叶皂苷钠 四氯化碳 肝纤维化 肝星状细胞 细胞凋亡 4EBP1 

分 类 号：R-332[医药卫生] R282.71