肉苁蓉苯乙醇总苷对压力超负荷大鼠心肌肥厚及PI3K/PKB信号通路的影响  

Effects of Cistanche Phenylethanol Glycosides on the cardiac hypertrophyand PI3K/PKB signaling pathway in rats with pressure overload

作　　者：樊琼玲 刘涛[2] 俞金秀 马正文 张石蕾 由淑萍[1] FAN Qiong-ling;LIU Tao;YU Jin-xiu;MA Zheng-wen;ZHANG Shi-lei;YOU Shu-ping(College of Nursing,Xinjiang Medical University,Urumqi 830011,China;College of Public Health,Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学护理学院,新疆乌鲁木齐830011 [2]新疆医科大学公共卫生学院,新疆乌鲁木齐830011

出　　处：《中国药理学通报》2020年第4期583-588,共6页Chinese Pharmacological Bulletin

基　　金：新疆维吾尔自治区省部共建中亚高发病成因与防治国家重点实验室开放课题(No SKL-HIDCA-2018-3);新疆维吾尔自治区高校自然科学项目(XJEDU2019Y027)。

摘　　要：目的探讨肉苁蓉苯乙醇总苷(CPhGs)对压力超负荷大鼠心肌肥厚的抑制作用及其可能的作用机制。方法♂SPF级SD大鼠70只,随机分为空白组(Con)、假手术组(Sham)、模型组(Mod)、阳性药对照组(Vst)、CPhGs 125、250、500 mg·kg^-1组。检测心脏超声指标、心脏体质量指数(HWI)、心肌组织病理学变化、心肌细胞面积(AMC),Elisa法检测血浆ET-1、BNP水平,Western blot法检测p-PI3K、PI3K、p-PKB、PKB蛋白表达变化。结果与Mod组相比,CPhGs不同剂量给药后,LVPWT、HWI、血浆ET-1和BNP、AMC均有不同程度下降,LVEDD、LVEF、LVFS以及心肌组织p-PI3K、p-PKB蛋白表达有不同程度的增加,其中CPhGs 250、500 mg·kg^-1组相比于Mod组各指标有明显差异(P<0.05或0.01);与Vst组相比,CPhGs 500 mg·kg^-1组各指标,没有明显差异。结论CPhGs对压力超负荷引起的大鼠心肌肥厚具有抑制作用,其作用可能与PI3K/PKB信号通路的激活有关。Aim To investigate the inhibitory effect of cistanche phenylethanol glycosides(CPhGs)on cardiac hypertrophy in rats caused by pressure overload and its related mechanism.Methods Male SD rats(n=70)were randomly divided into control group(Con),sham operation group(Sham),model group(Mod),positive control group(Vst),and different CPhGs dosage(125,250,500 mg·kg^-1)groups.Cardiac ultrasound indexes,heart-weight to body-weight index(HWI),cardiac histopathological changes,and the area of myocardical cells(AMC)were detected.Plasma ET-1 and BNP levels were detected by Elisa,and protein expressions of phosphorylated PI3K(p-PI3K),PI3K,phosphorylated PKB(p-pKB)and PKB were detected by Western blot.Results Compared with Mod group,LVPWT,HWI,plasma ET-1,BNP and AMC decreased to different degrees.LVEDD,LVEF,LVFS,the protein expressions of myocardial tissues p-PI3K and p-PKB increased to different degrees in CPhGs groups.Moreover,the indexes of CPhGs 250 and 500 mg·kg^-1 groups were significantly improved compared to those of Mod group(P<0.05 or 0.01).Compared with Vst group,there were no significant difference in CPhGs 500 mg·kg^-1 group.Conclusions CPhGs could inhibit cardiac hypertrophy in rats induced by pressure overload,which might be related to the activation of PI3K/PKB signaling pathway.

关 键 词：肉苁蓉苯乙醇总苷 压力超负荷 心肌肥厚 PI3K/PKB 腹主动脉缩窄术 磷酸化 

分 类 号：R-332[医药卫生] R284.1