蒟子的化学成分研究  

Chemical constituents of Piper yunnanense

作　　者：张潘华 杨珺[1] 罗吉凤[1] 夏梦媛 龙春林[2] 王俐[3] 王跃虎[1] ZHANG Pan-hua;YANG Jun;LUO Ji-feng;XIA Meng-yuan;LONG Chun-lin;WANG Li;WANG Yue-hu(Key Laboratory of Economic Plants and Biotechnology,and Yunnan Key Laboratory for Wild Plant Resource,Kunming Institute of Botany, Chinese Academy of Sciences,Kunming 650201, China;Key Laboratory of Ethnomedicine (Minzu University of China),Ministry of Education,Beijing 100081,China;College of Horticulture and Landscape,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]中国科学院昆明植物研究所资源植物与生物技术重点实验室及云南省野生资源植物研发重点实验室,昆明650201 [2]中央民族大学民族医药教育部重点实验室,北京100081 [3]云南农业大学园林园艺学院,昆明650201

出　　处：《天然产物研究与开发》2020年第2期232-238,256,共8页Natural Product Research and Development

基　　金：民族医药教育部重点实验室(中央民族大学)开放课题(KLEM-KF2018Z01)。

摘　　要：采用色谱柱层析及半制备高效液相色谱的分离方法,以及质谱和核磁共振波谱的鉴定方法,从蒟子全株中分离、鉴定出了10个化合物,其中4个为新的酰胺类成分,即蒟子酰胺A^D(1~4)。已知化合物包括3个二肽,即短蒟酰胺C^E(5~7),以及3个酰胺,即(E)-N-(3,4,5-三甲氧基肉桂酰基)四氢吡咯(8)、(E)-N-(5-甲氧基-3,4-亚甲二氧基肉桂酰基)四氢吡咯(9)和(Z)-N-(3,4,5-三甲氧基肉桂酰基)四氢吡咯(10)。采用MTS法测试了4个新化合物对5种肿瘤细胞株的体外细胞毒活性,蒟子酰胺A(1)对人白血病HL-60细胞(IC50=10.99μM)、人肝癌SMMC-7721细胞(IC50=17.10μM)、人肺癌A-549细胞株(IC50=7.93μM)、人乳腺癌MCF-7细胞(IC50=16.63μM)和人结肠癌SW480细胞(IC50=9.16μM)的生长有抑制活性。Through column chromatography and semi-preparative high performance liquid chromatography(HPLC),10 compounds were isolated from the whole plants of Piper yunnanense.These compounds including four new amides,namely piperunnamides A-D(1-4),along with three known dipeptides,pipermullamides C-E(5-7),and three known amides,(E)-N-(3,4,5-trimethoxycinnamoyl)pyrrolidine(8),(E)-N-(5-methoxy-3,4-methylenedioxycinnamoyl)pyrrolidine(9),and(Z)-N-(3,4,5-trimethoxycinnamoyl)pyrrolidine(10).Their structures were elucidated by MS and NMR spectra.The cytotoxic activity against five tumor cell lines of these new compounds was evaluated by an MTS assay.The result showed that piperunnamide A(1)exhibited growth inhibitory activity against human leukemia HL-60(IC50=10.99μM)cell line,human liver cancer SMMC-7721(IC50=17.10μM)cell line,human lung cancer A-549 cell line(IC50=7.93μM),human breast cancer MCF-7 cell line(IC50=16.63μM),and human colon cancer SW480 cell line(IC50=9.16μM).

关 键 词：胡椒科 蒟子 酰胺 细胞毒 

分 类 号：Q946[生物学—植物学]