低剂量紫杉醇联合沙利度胺抑制小鼠S-180肉瘤血管生成的研究  

Low-dose paclitaxel combined with thalidomide inhibits angiogenesis in mice bearing S180 sarcoma

作　　者：徐聂 白云丹 罗婷 段萍[3] Nie Xu;Yundan Bai;Ting Luo;Ping Duan(The Second Department of Oncology,Integrated TCM&Western Medicine Hospital Affiliated to Chengdu University of TCM,Chengdu First People's Hospital,Chengdu 610000,China;Department of Otolaryngology,Integrated TCM&Western Medicine Hospital Affiliated to Chengdu University of TCM,Chengdu First People's Hospital,Chengdu 610000,China;The First Department of Oncology,Integrated TCM&Western Medicine Hospital Affiliated to Chengdu University of TCM,Chengdu First People's Hospital,Chengdu 610000,China)

机构地区：[1]成都中医药大学附属中西医结合医院、成都市第一人民医院肿瘤二科,成都市610000 [2]成都中医药大学附属中西医结合医院、成都市第一人民医院耳鼻喉科,成都市610000 [3]成都中医药大学附属中西医结合医院、成都市第一人民医院肿瘤一科,成都市610000

出　　处：《中国肿瘤临床》2020年第1期7-11,共5页Chinese Journal of Clinical Oncology

摘　　要：目的:探讨低剂量紫杉醇联合沙利度胺对小鼠S-180肉瘤血管生成的作用。方法:建立S-180移植瘤小鼠模型,将40只荷瘤小鼠随机分为4组。A组:生理盐水组(对照组);B组:紫杉醇组,腹腔内注射20 mg/kg,每周3次,连续2周;C组:沙利度胺组,200 mg/kg灌胃,每周3次,连续2周;D组:紫杉醇+沙利度胺组,腹腔内注射紫杉醇(20 mg/kg)和沙利度胺(200 mg/kg)灌胃,每周3次,连续2周。观察瘤重、抑瘤率、血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达和微血管密度(microvessel density,MVD)计数。结果:与对照组比较,B、C、D组均能降低瘤重、减少VEGF的表达、降低MVD计数(P<0.05);D组分别与B、C组比较,VEGF的表达及MVD计数均低于B、C组,且差异具有统计学意义(P<0.05)。结论:小剂量紫杉醇联合沙利度胺能一定程度上抑制S180肉瘤血管的生长,两者具有协同作用。Objective:To investigate the effect of low-dose paclitaxel combined with thalidomide on angiogenesis in mice bearing S180 sarcoma.Methods:An S180 sarcoma-bearing mouse model was established.Forty tumor-bearing mice were randomly divided into four groups:A,the control group,administered normal saline;B,the paclitaxel group,administered 20 mg/kg paclitaxel by intraperitoneal injection three times per week for 2 weeks;C,the thalidomide group,administered 200 mg/kg thalidomide by gavage three times per week for 2 weeks;and D,the paclitaxel+thalidomide group,administered paclitaxel(20 mg/kg)and thalidomide(200 mg/kg)by intraperitoneal/intragastric administration three times per week for 2 weeks.Tumor weight,tumor inhibition rate,microvascular density(MVD),and vascular endothelial growth factor(VEGF)expression were measured.Results:Tumor weight,VEGF expression,and MVD were lower in groups B,C,and D(P<0.05)than in the control.VEGF expression and MVD were lower in group D than in groups B and C;these differences were statistically significant(P<0.05).Conclusions:Low-dose paclitaxel combined with thalidomide exerted an inhibitory effect on vascular growth in S180 sarcoma.

关 键 词：紫杉醇 沙利度胺 S-180 血管生成 

分 类 号：R738.6[医药卫生—肿瘤]