PROX1-AS1和PRICKLE2-AS1基因多态性与中国汉族人群2型糖尿病的相关性  

Correlation Study of Polymorphisms in PROX1-AS1 and PRICKLE2-AS1 Genes with T2DM in Chinese Han Population

作　　者：陶文玉[1] 李传印[2] 杨莹[1] 杨曼[1] 王晓苓[1] 角铭 何思琦 洪超[2] 李奕平[1] TAO Wenyu;LI Chuanyin;YANG Ying;YANG Man;WANG Xiaoling;JIAO Ming;HE Siqi;Hong Chao;LI Yiping(Department of Endocrinology and Metabolism,the Second People Hospital of Yunnan Province&the Fourth Affiliated Hospital of Kunming Medical University,Kunming 650021,Yunnan,China;Institute of Medical Biology,Chinese Academy of Medical Sciences&Peking Union Medical College,Kunming 650118,Yunnan,China)

机构地区：[1]云南省第二人民医院&昆明医科大学第四附属医院内分泌代谢科,云南昆明650021 [2]中国医学科学院&北京协和医学院,医学生物学研究所,云南昆明650118

出　　处：《贵州医科大学学报》2020年第2期169-173,共5页Journal of Guizhou Medical University

基　　金：国家自然科学基金项目(31660313);云南省科技厅-昆明医科大学应用基础研究联合专项[2019FE001(-092)和2013FB181];云南省中青年学术带头人后备人才项目(2018HB047);云南省高层次卫生计生技术人才培养项目(D-2017040)。

摘　　要：目的:探讨两个长链非编码RNA(lncRNA)基因(PROX1-AS1和PRICKLE2-AS1)中的单核苷酸多态性位点(SNP)与中国汉族人群2型糖尿病(T2DM)的相关性。方法:随机选取784名T2DM患者作为T2DM组,846名非糖尿病个体作为对照组;采用质谱法对lncRNA基因PROX1-AS1和PRICKLE2-AS1基因中的多态性位点rs2075423和rs12497268进行基因分型,并分析其与中国汉族人群T2DM的相关性。结果:结果显示PROX1-AS1基因中的多态性位点rs2075423等位基因在T2DM组和对照组中的分布频率差异有统计学意义,该位点等位基因G可能是T2DM的风险性因素(P<0.001,OR=1.394,95%CI为1.153~1.686),而PRICKLE2-AS1基因中的多态性位点rs12497268的基因型频率及等位基因频率在T2DM组和对照组中分布频率的差异无统计学意义(P>0.05);共显性遗传模式的分析结果显示,PROX1-AS1基因中多态性位点rs2075423基因型G/G相对于基因型G/T+T/T来说可能是T2DM发生的风险因素(P<0.001,OR=1.488,95%CI为1.197~1.851);PRICKLE2-AS1基因中多态性位点rs12497268基因型G/G相对于基因型G/C+C/C来说可能是T2DM发生的风险因素(P=0.027,OR=1.260,95%CI为1.027~1.545)。结论:PROX1-AS1和PRICKLE2-AS1基因中的多态性位点rs2075423和rs12497268可能与中国汉族人群T2DM发病风险相关。Objective:To investigate the correlation of single nucleotide polymorphisms(SNPs)(rs2075423 and rs12497268)in two long non-coding RNA(lncRNA)genes(PROX1-AS1 and PRICKLE2-AS1)with Type 2 diabetes(T2DM)in Chinese Han population.Methods:784 patients with T2DM and 846 subjects without diabetes mellitus were divided into T2DM group and control group respectively.rs2075423 in PROX1-AS1 gene and rs12497268 in PRICKLE2-AS1 gene were genotyped by the Mass Apectrometry.Then,their correlation with T2DM was analyzed.Results:The results showed that the distribution frequency difference of polymorphic site rs2075423 allele of PROX1-AS1 gene in T2DM group and control group were statistically significant,G allele of rs2075423 in PROX1-AS1 gene might be the risk factor of T2DM(P<0.001,OR=1.394;95%CI:1.153~1.686).While the allelic and genotypic frequency of rs12497268 in PRICKLE2-AS1 gene showed no significant difference between T2DM and control groups(P>0.05).Moreover,the codominant inheritance pattern analysis showed that G/G genotype of rs2075423 might be the risk factor of T2DM compared with G/T+T/T genotype(P<0.001,OR=1.488;95%CI:1.197~1.851).Similarly,G/G genotype of rs12497268 might be the risk factor of T2DM(P=0.027,OR=1.260;95%CI:1.027~1.545)compared with G/C+C/C genotype.Conclusion:Results revealed the two lncRNA SNPs(rs2075423 in PROX1-AS1 gene and rs12497268 in PRICKLE2-AS1 gene)may be correlated with T2DM recurrence risk in Chinese Han population.

关 键 词：糖尿病 2型 基因型 多态性 单核苷酸 遗传相关性研究 长链非编码RNA 

分 类 号：R394.3[医药卫生—医学遗传学]