circRNA在原发性肝细胞癌中诊断应用的Meta分析  

A Meta-analysis of the Diagnostic Utility of CircRNAs in Hepatocellular Carcinoma

作　　者：冯亚浩 张亚文 马鹏飞 郑幼伟[1] FENG Yahao;ZHANG Yawen;MA Pengfei;ZHENG Youwei(Department of Hepatological Surgery, The First Affiliated Hospital, and College of Clinical Medicine of Henan Universityof Science and Technology, Luoyang 471003, China;Department of Histology and Embryology,Ningxia Medical University, Yinchuan 750004, China)

机构地区：[1]河南科技大学临床医学院,河南科技大学第一附属医院肝胆外科,河南洛阳471000 [2]宁夏医科大学基础医学院组织与解剖学系,宁夏银川750004

出　　处：《标记免疫分析与临床》2020年第3期377-382,共6页Labeled Immunoassays and Clinical Medicine

摘　　要：目的综合评价环形RNA(circular RNA,circRNA)在原发性肝细胞癌(hepatocellular carcinoma,HCC)诊断中的综合效能,为HCC的早期诊断提供依据。方法检索PubMed、EMBASE、知网(CNKI)等在线数据库搜集circRNA诊断HCC的相关文献;由2名研究成员独立筛选文献、提取资料,评价纳入研究的偏倚风险后,通过STATA 12.0软件合并诊断效应指标,并进行亚组分析、灵敏度检验和Meta回归分析探讨研究异质性。结果共纳入11篇文献,包含880例患者。荟萃分析结果显示,circRNA诊断CRC和非肿瘤对照的合并灵敏度为0.75(95%CI:0.73~0.78),特异性为0.78(95%CI:0.75~0.81),曲线下面积(AUC)为0.86。亚组分析结果显示,基于癌组织的异常circRNA检测诊断HCC的效能高于血浆(AUC:0.89vs.0.74);circRNA表达检测用于区分HCC和癌旁的价值优于区分HCC和肝炎/肝硬化(AUC:0.89vs.0.74);检测HCC中表达升高的circRNA表达谱诊断HCC的价值高于表达下调的circRNA(AUC:0.90vs.0.81)。结论circRNA可作为较好的分子标志物用于辅助HCC的临床诊断。Objective To comprehensively assess the diagnostic efficacy of circular RNA(circRNA)expression pattern in confirmed hepatocellular carcinoma(HCC),so to provide the evidence for the early detection of HCC.Methods The online databases including PubMed,EMBASE,and CNKI were searched for eligible studies.Two investigators were arranged to screen literatures and extract the data independently.The diagnostic effects were combined using STATA 12.0 software after an evaluation of the risk and bias among all enrolled studies.Subgroup study,sensitivity analysis and meta-regression test were applied to fully assess the potential sources of study heterogeneity.Results A total of 11 eligible studies were included,comprising 880 HCC cases.Results of the meta-analysis showed that circRNAs harbored a combined sensitivity of 0.75(95%CI:0.73-0.78),specificity of 0.78(95%CI:0.75-0.81),and AUC(area under the curve)of 0.86 in differentiating HCC cases from non-cancer controls.Subgroup study showed that tissue-sourced circRNA testing achieved higher performance in detecting HCC than plasma-sourced circRNAs(AUC:0.89 vs.0.74);also the detection of circRNAs expression yielded higher diagnostic value in differentiating HCC from non-cancer controls than the efficacy in differentiating HCC from hepatitis/cirrhosis(AUC:0.89 vs.0.74);and elevated circRNAs showed an efficacy superior to down-regulated circRNAs in confirming HCC(AUC:0.90 vs.0.81).Conclusion CircRNAs can be considered as promising molecular biomarkers to aid the diagnosis of HCC.

关 键 词：环形RNA circRNA 肝细胞癌 诊断 META分析 

分 类 号：R735.7[医药卫生—肿瘤]