miRNA、CEA及Monototal在非小细胞肺癌诊断中的价值  

Value of miRNA,Carcinoembryonic Antigen and Monototal in Diagnosis of Non-Small Cell Lung Cancer

作　　者：孙菲[1] 王刚[1] 张金仿[1] SUN Fei;WANG Gang;ZHANG Jinfang(Center of Clinical Laboratory,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院临床检验中心,北京100050

出　　处：《医学综述》2020年第7期1429-1433,共5页Medical Recapitulate

摘　　要：目的探讨微RNA(miRNA)、癌胚抗原(CEA)和Monototal在非小细胞肺癌中的表达及意义。方法选取2017年10月至2018年10月于首都医科大学附属北京友谊医院检查确诊并进行手术治疗的50例非小细胞肺癌患者,取所有患者手术切除癌组织及距离癌组织5 cm以上的癌旁正常组织制作标本,采用酶联免疫吸附法检测miR-21、miR-146a、CEA和Monototal的表达,并对四项指标与临床病理资料的关系、四项指标之间的相关性、四项指标单独检测和联合检测的诊断效能进行分析。结果癌组织中miR-21、CEA和Monototal水平显著高于癌旁组织[2.37±0.29比1.31±0.64,(6.33±3.64)μg/L比(2.34±0.87)μg/L,(54.68±11.59)U/L比(5.62±1.25)U/L],miR-146a水平显著低于癌旁组织(0.45±0.27比1.24±0.31)(P<0.05)。Ⅰ+Ⅱ期、未及浆膜非小细胞肺癌患者的miR-21、CEA和Monototal表达水平低于Ⅲ+Ⅳ期、侵及浆膜非小细胞肺癌患者,miR-146a表达水平高于Ⅲ+Ⅳ期、侵及浆膜非小细胞肺癌患者(P<0.05);有淋巴结转移、肿瘤体积≥5 cm^3、低分化、中分化非小细胞肺癌患者的miR-21、CEA和Monototal表达水平高于无淋巴结转移、肿瘤体积<5 cm^3、高分化非小细胞肺癌患者,miR-146a表达水平低于无淋巴结转移、肿瘤体积<5 cm^3、高分化非小细胞肺癌患者(P<0.05)。miR-21与CEA和Monototal在非小细胞肺癌中的表达呈正相关(r=0.703,P=0.030;r=0.706,P=0.026),而与miR-146a的表达呈负相关(r=-0.710,P=0.029)。CEA单独检测的灵敏度、特异度、准确度、阳性预测值、阴性预测值显著高于miR-21、miR-146a与Monototal单独检测;四项指标联合检测的灵敏度、特异度、准确度、阳性预测值、阴性预测值显著高于四项指标单独检测。结论miR-21、miR-146a、CEA和Monototal在非小细胞肺癌中表达异常,且四项指标联合诊断效能较高,对非小细胞肺癌的临床诊断具有重要意义。Objective To explore the expression and significance of microRNA(miRNA),carcinoembryonic antigen(CEA)and Monototal in non-small cell lung cancer(NSCLC).Methods Fifty patients with NSCLC diagnosed and treated in Beijing Friendship Hospital,Capital Medical University from Oct.2017 to Oct.2018 were included in the study,and all of the patients received resection.The cancer tissue and paracancerous normal tissues more than 5 cm away from cancer tissue were obtained for making specimen.The expression of miR-21,miR-146a,CEA and Monototal were detected by enzyme-linked immunosorbent assay,and the relationship between the four indexes and clinical pathological data,the correlation between the four indexes,the diagnostic efficacy of the four indexes detected separately and jointly were analyzed.Results The levels of miR-21,CEA and Monototal in the cancer tissues were significantly higher than those in the paracancerous tissue[2.37±0.29 vs 1.31±0.64,(6.33±3.64)μg/L vs(2.34±0.87)μg/L,(54.68±11.59)U/L vs(5.62±1.25)U/L],and the level of miR-146a was significantly lower than that of the paracancerous tissue(0.45±0.27 vs 1.24±0.31)(P<0.05).The expression levels of miR-21,CEA and Monotal in patients with stageⅠ+Ⅱ,non serous NSCLC were lower than those in patients with stageⅢ+Ⅳ,serous NSCLC,and miR-146a was higher than those in patients with stageⅢ+Ⅳ,serous NSCLC(P<0.05);the expression levels of miR-21,CEA and Monototal in patients with lymph node metastasis,tumor volume≥5 cm^3,and low-differentiated and medium-differentiated NSCLC were higher than those in patients without lymph node metastasis,tumor volume<5 cm^3,and highly differentiated NSCLC,while the expression levels of miR-146a were lower than those in patients without lymph node metastasis,tumor volume<5 cm^3 and highly differentiated NSCLC(P<0.05).The expression of miR-21 was positively correlated with CEA and Monototal in NSCLC(r=0.703,P=0.030;r=0.706,P=0.026),but negatively correlated with miR-146a expression(r=-0.710,P=0.029).The sensitivity,s

关 键 词：非小细胞肺癌 微RNA 癌胚抗原 Monototal 

分 类 号：R734.2[医药卫生—肿瘤]