NGF-BMSCs移植对AD动物模型症状改善及对脑组织中凋亡相关基因Bcl-2、Bax水平的影响  

Effect of NGF-BMSCs transplantation on the improvement of symptoms in AD animal model and the levels of apoptosis-related genes Bcl-2 and Bax in brain tissue

作　　者：梁金[1] 谷新医[1] 蒋志宏[1] 马强[1] LIANG Jin;GU Xin-yi;JIANG Zhi-hong;MA Qiang(The Second Department of Neurology,Affiliated Zhongshan Hospital of Dalian University,Liaoning Province,Dalian 116000,China)

机构地区：[1]大连大学附属中山医院神经内二科,辽宁大连116000

出　　处：《中国当代医药》2020年第8期4-7,共4页China Modern Medicine

基　　金：国家自然科学基金青年科学基金项目(81301089)。

摘　　要：目的 探讨神经生长因子-骨髓基质干细胞(NGF-BMSCs)移植对阿尔兹海默病(AD)动物模型症状改善及对脑组织中凋亡相关基因B淋巴细胞瘤-2(Bcl-2)、Bax水平的影响.方法 将50只AD大鼠随机分为5组,分别为对照组、盐水注射组、模型组、BMSCs移植组和NGF-BMSCs移植组,每组各10只.观察AD大鼠的行为学变化情况,检测大鼠海马区Bcl-2、Bax水平.结果 模型组和盐水注射组第1~6天的逃避潜伏期均短于对照组,差异有统计学意义(P<0.05);BMSCs移植组、NGF-BMSCs移植组第1~6天的逃避潜伏期均长于对照组、模型组和盐水注射组,差异有统计学意义(P<0.05);NGF-BMSCs移植组第1~6天的逃避潜伏期长于BMSCs移植组,差异有统计学意义(P<0.05).模型组、盐水注射组、BMSCs移植组、NGF-BMSCs移植组的Bcl-2、Bax水平均高于对照组,差异有统计学意义(P<0.05);盐水注射组和BMSCs移植组的Bcl-2水平均高于模型组,Bax水平均低于模型组,差异有统计学意义(P<0.05);BMSCs移植组和NGF-BMSCs移植组的Bcl-2均低于盐水注射组,差异有统计学意义(P<0.05);NGF-BMSCs移植组的Bcl-2水平低于BMSCs移植组,Bax水平高于盐水注射组、BMSCs移植组,差异有统计学意义(P<0.05).结论 NGF-BMSCs移植通过调节AD动物模型海马组织中Bcl-2、Bax表达水平抑制其神经细胞凋亡.Objective To investigate the effect of nerve growth factor-bone marrow stromal stem cells(NGF-BMSCs)transplantation on the improvement of symptoms in(AD)animal model and the levels of apoptosis-related genes B lymphocyte tumor-2(Bcl-2)and Bax in brain tissue.Methods Fifty AD rats were randomly divided into five groups,namely the control group,saline injection group,model group,BMSCs transplantation group and NGF-BMSCs transplantation group,with 10 rats in each group.The behavioral changes of AD rats were observed,and the levels of Bcl-2 and Bax in hippocampus of rats were detected.Results The escape latency of the model group and the saline injection group from the first day to the sixth day was shorter than that of the control group,with statistically significant differences(P<0.05).The escape latency of BMSCs transplantation group and NGF-BMSCs transplantation group from the first day to the sixth day was longer than that of the control group,model group and saline injection group,with statistically significant differences(P<0.05).The escape latency of the NGF-BMSCs transplantation group from the first day to the sixth day was longer than that of the BMSCs transplantation group,and the difference was statistically significant(P<0.05).The levels of Bcl-2 and Bax in the model group,saline injection group,BMSCs transplantation group,and NGF-BMSCs transplantation group were higher than those in the control group,and the differences were statistically significant(P<0.05).The Bcl-2 levels in the saline injection group and the BMSCs transplantation group were higher than those in the model group,the Bax levels were lower than those in the model group,and the differences were statistically significant(P<0.05).The levels of Bcl-2 in the BMSCs transplantation group and NGF-BMSCs transplantation group were lower than those in the saline injection group,and the differences were statistically significant(P<0.05).The Bcl-2 level in the NGF-BMSCs transplantation group was lower than that in the BMSCs transplantation group,the

关 键 词：神经生长因子基因-骨髓基质干细胞移植 阿尔兹海默病 动物模型 症状改善 凋亡基因 

分 类 号：R743[医药卫生—神经病学与精神病学]