过磷酸化Tau蛋白在神经元逆行性退变中的表达  

Expression of hyperphosphorylated Tau protein in retrograde degeneration of neurons

作　　者：陶善春 潘筱云 柳晨 糜菁熠 Tao Shanchun;Pan Xiaoyun;Liu Chen;Mi Jingyi(Department of Hand Surgery,Wuxi No.9 Hospital Affiliated to Suzhou University,Wuxi 214062,China)

机构地区：[1]苏州大学附属无锡九院手外科,无锡241062

出　　处：《中华手外科杂志》2020年第2期126-130,共5页Chinese Journal of Hand Surgery

基　　金：上海市周围神经显微外科重点实验室,卫生部手功能重建重点实验室基金(17DZ2270500)。

摘　　要：目的探讨颈7神经切断后发生的脊髓中枢神经元逆行性退变中过磷酸化Tau蛋白的表达情况,为外周神经损伤后中枢神经元逆行性退变保护提供新思路。方法将成年雌性SD大鼠36只随机分为3组(假手术组、切断组、切断+药物干预组),其中切断组和干预组的动物施行双侧颈7神经切断造模,干预组予每天腹腔注射1 mmol/kg氯化锂。2周、4周后取大鼠颈7节段脊髓,通过HE染色、流式细胞技术来分析神经细胞的凋亡情况,Western Blot分析Tau蛋白及相关蛋白表达水平。结果HE染色及流式细胞学检测显示2周时切断组的细胞凋亡程度小于4周时的凋亡程度;Western Blot结果提示随着周数的增加,总Tau蛋白的量因颈7神经的切断而下降,磷酸化的Tau蛋白的量的比例上升,差异具有统计学意义。予氯化锂后,凋亡的程度及磷酸化的Tau蛋白的比例均发生下降,差异具有统计学意义。结论Tau蛋白的过磷酸化机制存在于颈7神经切断术后发生的脊髓中枢神经元逆行性退变的过程中;氯化锂能减轻逆行性退变的程度。Objective To explore the expression of hyperphosphorylated Tau protein in the retrograde degeneration of spinal central neurons after cervical 7 nerve transection,so as to provide a new idea for the protection of retrograde degeneration of central neurons after peripheral nerve injury.Methods Thirty-six adult female Sprague-Dawley rats were randomly divided into three groups(sham operation group,transection group,transection+drug intervention group).The rats in transection group and transection+drug intervention group were treated with bilateral cervical 7 nerve cutting to make models,and the rats in transection+drug intervention group were treated with 1 mmol/kg lithium chloride intraperitoneally every day.After 2 and 4 weeks,the spinal cords of cervical 7 of the rats were taken,and the apoptosis of neurons was analyzed by HE staining and flow cytometry.The expression of Tau protein and related protein was analyzed by Western blot.Results HE staining and flow cytometry showed that the apoptotic degree of the transection group at 2 weeks was less than that at 4 weeks.The resutls of Western blot showed that as time went on,the amount of total Tau protein decreased due to the transection of cervical 7 nerve,and the proportion of phosphorylated Tau protein increased.The difference was statistically significant.After lithium chloride administration,the degree of apoptosis and the proportion of phosphorylated Tau protein decreased significantly.Conclusion The mechanism of Tau protein hyperphosphorylation lies in the process of retrograde degeneration of spinal cord central neurons after cervical 7 transection,and lithium chloride can reduce the degree of retrograde degeneration.

关 键 词：TAU蛋白质类 臂丛 神经元逆行性退变 过磷酸化 

分 类 号：R74[医药卫生—神经病学与精神病学]