CD44和RHAMM在胃肠肿瘤中的表达及其临床意义  

Detection of CD44 and RHAMM Expression and Prognosis in Gastrointestinal Tumors by Real-time Fluorescence Quantitative PCR

作　　者：王建芳 康乐 宋红杰 WANG Jianfang;KANG Le;SONG Hongjie(Zhumadian Central Hospital,Zhumadian,463000)

机构地区：[1]河南省驻马店市中心医院,463000

出　　处：《实用癌症杂志》2020年第4期530-533,共4页The Practical Journal of Cancer

基　　金：2018年度河南省医学科技攻关项目(编号:2018020504)。

摘　　要：目的研究实时荧光定量PCR检测CD44和RHAMM在胃肠肿瘤中的表达及其临床意义。方法随机选取胃肠肿瘤患者95例,将肿瘤组织及其周边5cm以上的正常组织取出来,保存在液氮中。进行总RNA抽提与cDNA合成、逆转录-PCR、CD44、RHAMM GAPDH质粒制备、实时PCR。结果实时荧光定量PCR检测CD44、RHAMM在胃肠肿瘤组织中的表达均显著高于正常组织(P<0.05)。CD44和RHAMM在胃肠肿瘤的表达显著相关(P<0.05),其中肠道、有淋巴结转移CD44和RHAMM均呈显著的正相关关系(P<0.05),但胃部、无淋巴结转移CD44和RHAMM均无相关性(P>0.05)。结论实时荧光定量PCR检测CD44和RHAMM在胃肠肿瘤组织中的表达均高于正常组织,且具有相关性,能够对肿瘤淋巴结转移情况进行判定参考。Objective To study the expression and prognosis of CD44 and RHAMM in gastrointestinal tumors by real-time fluorescence quantitative PCR.Methods 95 patients with gastrointestinal tumors were randomly selected.The normal tissues above 5 cm around the tumors were taken out and stored in liquid nitrogen.Total RNA extraction and DNA synthesis,reverse transcription-PCR,CD44,RHAMM GAPDH plasmid preparation,real-time PCR were carried out.Results The expression of CD44 in gastrointestinal tumors was significantly higher than that in normal tissues by real-time fluorescence quantitative PCR(P<0.05).The expression of RHAMM in gastrointestinal tumors was significantly higher than that in normal tissues by real-time fluorescence quantitative PCR(P<0.05).CD44 and RHAMM were positively correlated in intestinal tract,lymph node metastasis(P<0.05),but not in stomach and non-lymph node metastasis(P>0.05).Conclusion The expression of CD44 and RHAMM in gastrointestinal cancer tissues detected by real-time fluorescence quantitative PCR is higher than that in normal tissues,and has correlation.It can provide effective basis for clinical diagnosis of lymph node metastasis.

关 键 词：实时荧光定量PCR CD44 RHAMM 胃肠肿瘤 表达 预后 

分 类 号：R735.2[医药卫生—肿瘤] R735.3[医药卫生—临床医学]