肺癌患者血清miR-200a的表达水平及意义  

Expression Level and Significance of Serum Mir-200a in Lung Cancer Patients

作　　者：戚永超[1] 缪劲[1] 郑琳[1] 张爱平[1] QI Yongchao;MIAO Jin;ZHENG Lin(The Affiliated Nanjing Hospital of Nanjing Medical University(Nanjing First Hospital),Nanjing,210000)

机构地区：[1]南京医科大学附属南京医院,南京市第一医院,210000

出　　处：《实用癌症杂志》2020年第4期556-560,共5页The Practical Journal of Cancer

摘　　要：目的分析肺癌患者血清miR-200a的表达水平及意义。方法选取肺癌患者50例为研究对象,取其癌组织与癌旁组织,并选择人肺癌细胞系A549与永生化支气管上皮细胞株16HBE,采用逆转录-聚合酶链反应(RT-PCR)法检测肺癌细胞及组织中miR-200a表达水平,应用脂质体转染法将miR-200a mimics与阴性对照序列(miR-NC)分别转染A549,以CCK-8实验和平板克隆形成实验检测细胞增殖情况,以Transwell实验检测细胞迁移能力,应用流式细胞技术检测细胞凋亡情况,并分析肺癌患者miR-200a表达水平与临床病理参数的关系。结果肺癌患者癌组织中miR-200a表达水平低于癌旁组织(P<0.05),A549细胞系中miR-200a表达水平也低于HBE细胞系,差异有统计学意义(P<0.05);CCK-8实验显示,miR-200a mimics第3 d、4 d、5 d的OD值低于miR-NC,平板克隆形成实验发现miR-200a mimics克隆形成率低于miR-NC,Transwell实验显示miR-200a mimics穿膜细胞数少于miR-NC,流式细胞技术检测显示,miR-200a mimics凋亡率高于miR-NC(P<0.01);有淋巴结转移、临床分期Ⅲ期者miR-200a表达水平低于无淋巴结转移、临床分期Ⅱa~Ⅱb期者(P<0.05)。结论肺癌患者中miR-200a呈低表达,与淋巴结转移有一定关联,且体外实验也发现miR-200a可抑制肺癌细胞A549的增殖、迁移并促进其凋亡,可将其作为肺癌治疗的潜在靶点。Objective To analyze the expression levels and significance of serum miR-200a in lung cancer patients.Methods A total of 50 patients with lung cancer were selected as the research subjects.Their cancer tissues and adjacent tissues were collected.The human lung cancer cell line A549 and immortalized bronchial epithelial cell line 16HBE were selected for detecting the expression levels of miR-200a in lung cancer cells and tissues by reverse transcription-polymerase chain reaction(RT-PCR).The miR-200a mimics and negative control sequence(miR-NC)were respectively transfected into A549 by lipofection.Cell proliferation,migration ability and apoptosis were detected by CCK-8 assay and plate colony formation assay,Transwell assay and flow cytometry,respectively.The relationship between miR-200a expression levels and clinicopathological parameters in lung cancer patients was analyzed.Results The expression levels of miR-200a in cancer tissues of lung cancer patients were lower than those in adjacent tissues(P<0.05).The expression levels of miR-200a in A549 cell lines were lower than those of HBE cell lines(P<0.05).CCK-8 assay showed that OD values of miR-200a mimics on the 3rd,4th and 5th day were lower than those of miR-NC.The plate colony formation assay found that clone formation rate of miR-200a mimics was lower than that of miR-NC.Transwell assay showed number of transmembrane cells of miR-200a mimics was less than that of miR-NC.Flow cytometry showed that apoptotic rate of miR-200a mimics was higher than that of miR-NC(P<0.01).The expression levels of miR-200a in patients with lymph node metastasis and clinical staging at stage Ⅲ were lower than those without lymph node metastasis and clinical staging at stage Ⅱ a toⅡb(P<0.05).Conclusion The expression of miR-200a is low in lung cancer patients,which is associated with lymph node metastasis.In vivo experiment find that miR-200a can inhibit proliferation and migration of lung cancer cell A549 and promote apoptosis.It can be used as a potential target for lung c

关 键 词：肺癌 miR-200a MIRNA A549 

分 类 号：R734.2[医药卫生—肿瘤]