Callus Cultures Of Beans Infected With Virus As A Model For Testing Antiviral Compaunds  

作　　者：Kovalenko Oleksiy Kyrychenko Angelina Kovalenko Olena 

机构地区：[1]Zobolotny Institute of Microbiology and Virology NAS of Ukraine,Kyiv,03143,Ukraine

出　　处：《Journal of Botanical Research》2019年第2期19-24,共6页植物研究（英文）

基　　金：supplied by the National Academy of Sciences of Ukraine(governmental registration number of work is 0110U001871).

摘　　要：In the work,bean callus raised from a leaves of Bean common mosaic virus infected bean plant was obtained and adapted for the testing of antiviral activity of liposomal glycan-glycolipid complexes.Ganoderma adspersum glucans and Pseudomonas spec.rhamnolipids were constituents of liposomal compaunds.It has been shown that under the long-term cultivation(up to 3 months)in the presence of a liposomal preparation containing(10-100 mg/l),the virus is eliminated from the tissue.This is evidenced by the absence of 391 bp sequence amplification product established by RT-PCR in the callus tissue,cultured on a medium containing the liposomal complex.The proposed model system is analogous to plant tumors and has obvious advantages over similar systems in vivo,since the callus growth is controlled and independent of environmental factors.

关 键 词：BEAN common MOSAIC virus(BCMV) BEAN yellow MOSAIC virus(BYMV) Reverse transcriptase polymerase chain reaction(RT-PCR) Plant tumors GLYCANS GLYCOLIPIDS Therapy of VIRUS diseases 

分 类 号：R51[医药卫生—内科学]