氮源对紫球藻和蓝隐藻胞外多糖及藻胆蛋白含量的影响  

Effects of Nitrogen Sources on Exopolysaccharide and Phycobiliprotein Contents of Porphyridium sp.and Chroomonas placoidea

作　　者：梁英[1] 胡乃霞 黄徐林 田传远 闫译允 王帅 LIANG Ying;HU Nai-Xia;HUANG Xu-Lin;TIAN Chuan-Yuan;YAN Yi-Yun;WANG Shuai(The Key Laboratory of Mariculture(Ocean University of China),Ministry of Education,Qingdao 266003,China;Guangdong Haid Group Co.,Ltd.,Guangzhou 511400,China;The Key Laboratory of Marine Bioactive Substance,the First Institute of Oceanography Ministry of Natural Resources,Qingdao 266061,China)

机构地区：[1]海水养殖教育部重点实验室(中国海洋大学),山东青岛266003 [2]广东海大集团股份有限公司,广东广州511400 [3]海洋活性物质与现代分析技术重点实验室(自然资源部第一海洋研究所,山东青岛266061

出　　处：《中国海洋大学学报（自然科学版）》2020年第5期31-39,共9页Periodical of Ocean University of China

基　　金：山东省重点研究发展计划项目(2017GHY15104);中央级公益性科研院所基本科研业务费专项(2017Q09);国家自然科学基金项目(41806201)资助。

摘　　要：本文研究了4种不同氮源(硝酸钠、氯化铵、脲和硝酸铵)对紫球藻(Porphyridium sp.)和蓝隐藻(Chroomonas placoidea)叶绿素荧光参数(PSII最大光能转化效率F v/F m、相对电子传递效率rETR、光化学淬灭系数qP、非光化学淬灭系数NPQ)、细胞密度、干重、胞外多糖和藻胆蛋白含量的影响。研究结果表明,有利于紫球藻和蓝隐藻生长和光合作用的最佳氮源是硝酸钠,在此条件下,实验结束时,2种微藻的F v/F m、rETR、qP、细胞密度和干重均达到最大值,而氯化铵则对其上述参数均有抑制作用。有利于紫球藻分泌胞外多糖和合成藻胆蛋白的最佳氮源分别是氯化铵和硝酸铵,在此条件下,该藻胞外多糖和藻胆蛋白含量分别达到干重的29.0%和17.5%;有利于蓝隐藻分泌胞外多糖和合成藻胆蛋白的最佳氮源分别是脲和硝酸铵,在此条件下,该藻胞外多糖和藻胆蛋白含量分别达到干重的17.5%和18.8%。综合考虑生物量和活性物质含量,获取2种微藻胞外多糖的较理想氮源是脲,获取2种微藻藻胆蛋白的较理想氮源是硝酸钠。该结果为2种微藻的培养及开发利用提供了理论依据。The chlorophyll fluorescence parameters(the maximal photochemical efficiency of PSII(F v/F m),the relative electron transport rate(rETR),the photochemical quenching(qP),the non-photochemical quenching(NPQ)),the cell density,the dry weight,the exopolysaccharide and phycobiliprotein contents of Porphyridium sp.and Chroomonas placoidea were investigated when sodium nitrate,ammonium chloride,urea and ammonium nitrate were used independently as the nitrogen source.The results indicated that the optimal nitrogen source for the growth and photosynthesis of Porphyridium sp.and C.placoidea was sodium nitrate.When sodium nitrate was used as the sole nitrogen source,the F v/F m,rETR,qP,cell density and dry weight of the two microalgae maximized at the end of experiment.In contrast,these parameters were suppressed when ammonium chloride served as the sole nitrogen source.The results showed that the best nitrogen sources for the exopolysaccharide secretion and phycobiliprotein synthesis in Porphyridium sp.were ammonium chloride and ammonium nitrate,respectively,while those in C.placoidea were urea and ammonium nitrate,respectively.When these two nitrogen sources were used,the contents of exopolysaccharide and phycobiliprotein in Porphyridium sp.were 29.0%and 17.5%of dry weight while those in C.placoidea were 17.5%and 18.8%,respectively.By considering comprehensively the biomass and active substance content,the ideal nitrogen source for obtaining exopolysaccharide from the two microalgae was urea while that for phycobiliprotein was sodium nitrate.These findings provided a theoretical basis for the culture and exploration of two microalgae.

关 键 词：紫球藻 蓝隐藻 氮源 叶绿素荧光参数 胞外多糖 藻胆蛋白 

分 类 号：S917.3[农业科学—水产科学] Q178.1[生物学—水生生物学]