CD151-MUT突变对肺腺癌细胞A549迁移的影响及机制  

Effect of CD151-MUT mutation on migration of lung adenocarcinoma cell line A549 and its mechanism

作　　者：杨秀婷 刘启功[2] 左萍[2] 刘正湘[2] 左后娟 YANG Xiuting;LIU Qigong;ZUO Ping;LIU Zhengxiang;ZUO Houjuan(Department of Cardiology of Binzhou Medical University Hospital,Binzhou 256600,Shandong,China;Department of Cardiology of Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Hubei,China)

机构地区：[1]滨州医学院附属医院心血管内科,山东滨州256600 [2]华中科技大学同济医学院附属同济医院心血管内科,湖北武汉430030

出　　处：《山东大学学报（医学版）》2020年第3期81-86,共6页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81873535)。

摘　　要：目的 研究CD151整合素结合缺陷突变体(CD151-MUT)突变对肺腺癌细胞A549迁移的影响及机制.方法 分别运用CD151、CD151-MUT和GFP质粒转染肺腺癌A549细胞,作为CD151组、CD151-MUT组和GFP组,同时设置Control组及Reagent组.改良的Boyden趋化小室法检测各组细胞的体外迁移能力.Western blot-ting方法检测各组细胞CD151蛋白、黏着斑激酶(FAK)及P130Cas蛋白的表达.结果 ①CD151组(2.23±0.22)和CD151-MUT组(2.15±0.17)CD151蛋白的表达较Control组(1.03±0.15)、GFP组(1.18±0.13)和Reagent组(1.22±0.11)均增高,5组均值差异有统计学意义(F=39.8,P<0.001),但两两多重比较显示CD151组和CD151-MUT组间差异无统计学意义(q=1.007,P=0.628).②CD151组较Control组、GFP组及Reagent组促进肿瘤细胞的迁移(F=15.3,P=0.007),而CD151-MUT组细胞的迁移能力较CD151组下降,差异有统计学意义(q=6.760,P=0.010).③CD151高表达促进p-FAK、p-P130Cas蛋白表达增加(F=16.3,P<0.001;F=10.9,P=0.001),而CD151-MUT组p-FAK、p-P130Cas蛋白表达水平较CD151组减弱(q=8.620,P=0.010;q=6.859,P=0.009).结论 CD151-MUT突变破坏CD151-整合素复合体形成,抑制肿瘤转移.CD151-α3/α6整合素复合体在肿瘤细胞迁移过程中发挥重要作用.Objective To study the effect of CD151-MUT on migration of lung adenocarcinoma cell line A549 and the mechanism involved.Methods pAAV-CD151,pAAV-CD151-MUT and pAAV-GFP were constructed and transfected into A549,and named as CD151 group,CD151-MUT group and GFP group.Meanwhile,Control group and Reagent group were set up.The migration of A549 was detected by modified Boyden chamber assay.Protein expression levels of CD151,p-focal adhesion kinase(FAK)and p-P130Cas were measured by Western blotting.Results①Compared with the Control group(1.03±0.15),GFP group(1.18±0.13)and Reagent group(1.22±0.11),the expression of CD151 protein was increased both in the CD151 group(2.23±0.22)and CD151-MUT group(2.15±0.17)with statistical differ-ence(F=39.8,P<0.001),but there was no significant difference of the CD151 protein expression between CDC151 and CDC151-CDC151-MUT groups(q=1.007,P=0.628).②The CD151 group significantly promoted cells migration compared with the Control group,GFP group and Reagent group(F=15.3,P=0.007).However,compared with the CD151 group,the migration and invasion of A549 cells were decreased in CD151-MUT group(q=6.760,P=0.010).③Overexpression of the CD151 protein could promote the expressions of p-FAK and p-P130Cas proteins(F=16.3,P<0.001;F=10.9,P=0.001),while the protein expressions were reduced in the CD151-MUT group compared with CD151 group(q=8.620,P=0.010;q=6.859,P=0.009).Conclusion CD151-MUT mutation impairs the formation of CD151-integrin and inhibits the tumor metastasis.The CD151-α3/α6 integrins complex is functionally important.

关 键 词：整合素 CD151 肿瘤转移 FAK-P130Cas 肺腺癌细胞A549 

分 类 号：R734.2[医药卫生—肿瘤]