血清miR-21在食管鳞癌中的表达及临床意义  

Serum miR-21 in squamous cell carcinoma of esophagus and its clinical significance

作　　者：郑志伟[1] 朱辉德[1] 方玫玫[2] 蔡晓锐[1] 朱志伟[1] 陈蕾[2] ZHENG Zhiwei;ZHU Huide;FANG Meimei;CAI Xiaorui;ZHU Zhiwei;Chen Lei(Department of Pharmacy,Cancer Hospital of Shantou University Medical College,Shantou Guangdong 515041,China;Department of Gastroenterology,Cancer Hospitalof Shantou University Medical College,Shantou Guangdong 515041,China)

机构地区：[1]汕头大学医学院附属肿瘤医院药学部,广东汕头515041 [2]汕头大学医学院附属肿瘤医院消化肿瘤内科,广东汕头515041

出　　处：《转化医学杂志》2020年第2期97-100,共4页Translational Medicine Journal

基　　金：2017年广东省医院药学研究基金资助项目(2017A16)。

摘　　要：目的检测血清miR-21在食管鳞癌患者中的表达及临床意义。方法收集2016年10月-2017年6月汕头大学医学院附属肿瘤医院食管鳞癌患者50例和健康体检者50例血清标本,提取miRNAs,通过实时荧光定量PCR检测miR-21表达水平,结合食管鳞癌患者临床资料进行统计分析。结果在食管鳞癌患者血清样本中,miR-21的表达水平明显高于健康对照组,差异比较具有统计学意义(P<0.001);miR-21的高表达与肿瘤远处器官转移及淋巴结转移呈正相关(P<0.001);临床分期为Ⅲ、Ⅳ期的患者血清miR-21表达水平明显高于Ⅰ、Ⅱ期患者(P<0.05);miR-21在胸中、下段食管鳞癌患者中的表达水平与胸上段食管鳞癌患者相比显著上调(P<0.05)。结论血清miR-21表达水平与食管鳞癌的发生、转移及临床分期密切相关,有望成为新的肿瘤标志物。Objective To detect the expression of serum miR-21 in patients with squamous cell carcinoma of the esophagus and to investigate the clinical significance of the expression of miR-21 in squamous cell carcinoma of the esophagus. Methods Fifty patients with squamous cell carcinoma of the esophagus were selected as the observation group from October 2016 to June 2017,50 healthy subjects were selected as the control group. Serum was collected from esophageal squamous cell carcinoma patients and healthy subjects. The expression of miR-21 was detected by fluorescent quantitative PCR. The clinical data of patients were collected and analyzed. Results The serum level of miR-21 in patients with squamous cell carcinoma of esophagus was significantly higher than that in healthy control(P < 0. 001). The high expression of miR-21 was also positively correlated with distant metastasis and lymph node metastasis(P < 0. 001). The serum level of miR-21 in patients with stage Ⅲ and Ⅳ was significantly higher than that in patients with stage Ⅰ and Ⅱ(P <0. 05). The expression level of miR-21 in patients with squamous cell carcinoma of the lower thoracic esophagus was significantly higher than that of the patients with squamous cell carcinoma of the upper thoracic region(P<0. 05). Conclusion The level of serum miR-21 is closely related to the occurrence,metastasis and clinical stage of esophageal squamous cell carcinoma,suggesting that miR-21 may be involved in carcinogenesis and progression of esophageal squamous cell carcinoma which may become a new tumor marker.

关 键 词：食管鳞癌 MIR-21 肿瘤标志物 

分 类 号：R735.1[医药卫生—肿瘤]