RLIP76和CD44在胃癌患者血清和组织中表达及其临床意义  

Expression of RLIP76 and CD44 in serum and tissues of patients withgastric cancer and their clinical significance

作　　者：万涛[1] 熊伟杰[1] 王云涛[1] WAN Tao;XIONG Weijie;WANG Yuntao(Department of Oncology,Chengdu Fifth People's Hospital,Chengdu Sichuan 611130,China)

机构地区：[1]成都市第五人民医院肿瘤内科,四川成都611130

出　　处：《转化医学杂志》2020年第2期101-105,共5页Translational Medicine Journal

摘　　要：目的探讨RLIP76和CD44在胃癌患者血清和组织中的表达及其临床意义。方法收集2014年3月-2016年5月于成都市第五人民医院行手术治疗的82例胃癌患者的临床资料,所有患者手术前后均留取外周血标本,采用酶联免疫法测定患者血清RLIP76及CD44水平的变化;术中留取癌组织及癌旁组织标本,采用免疫组化法测定胃癌组织RLIP76、CD44表达情况,分析RLIP76、CD44表达与胃癌生物学特点及预后的关系。结果术后2周,胃癌患者血清RLIP76、CD44表达水平降低,与术前3 d比较差异有统计学意义(t=18. 390,26. 291;均P<0. 05);胃癌患者癌组织RLIP76阳性率为50. 00%,CD44阳性率为58. 54%,患者癌组织RLIP76、CD44阳性率均高于癌旁组织(χ~2=48. 681,65. 063;均P<0. 05);组织分化程度为低分化、临床分期为Ⅲ~Ⅳ期、伴淋巴结转移的胃癌患者RLIP76阳性率、CD44阳性率高于组织分化程度为高、中分化、临床分期Ⅰ~Ⅱ期及不伴淋巴结转移的胃癌患者(P<0. 05);RLIP76阳性、CD44阳性胃癌患者生存时间短于阴性者,但Log-Rank检验差异无统计学意义(χ~2=2. 057,P=0. 151;χ~2=0. 452,P=0. 501);Cox分析显示淋巴结转移、临床分期、RLIP76阳性、CD44阳性均为影响胃癌预后的独立危险因素(P<0. 05)。结论胃癌患者血清及癌组织RLIP76、CD44水平呈异常高表达,且与胃癌组织分化程度、临床分期及淋巴结转移有关,是导致患者不良预后的独立危险因素。Objective To investigate the expression of RLIP76 and CD44 in serum and tissues of patients with gastric cancer and their clinical significance.Methods Clinical data of 82 patients with gastric cancer undergoing operation in the hospital from March 2014 to May 2016 were collected.Peripheral blood samples were collected from all patients before and after operation and changes in levels of serum RLIP76 and CD44 were determined by enzyme-linked immunosorbent assay(ELISA).The tumor tissue and adjacent tissue specimens were collected during surgery,and the expression of RLIP76 and CD44 in gastric cancer tissues was determined by immunohistochemistry.The relationship between expression of RLIP76 and CD44 and biological characteristics and prognosis of gastric cancer was analyzed.Results The levels of serum RLIP76 and CD44 in patients with gastric cancer were decreased in 2 weeks after operation.There were significant differences between the two groups,compared with those at 3 days before operation(t=18.390,26.291;all P<0.05).The positive rates of RLIP76 and CD44 in gastric cancer tissues(50.00%,58.54%)were higher than those in adjacent tissues(χ^2=48.681,65.063;all P<0.05).The positive rates of RLIP76 and CD44 were higher in patients with poorly differentiated gastric cancer,patients with gastric cancer at clinical stageⅢ-Ⅳand patients with lymph node metastasis than in patients with highly differentiated gastric cancer,patients with gastric cancer at clinical stageⅠ-Ⅱand patients without lymph node metastasis(P<0.05).The survival time of RLIP76-positive and CD44-positive patients with gastric cancer was shorter than that of negative patients,and there was no statistically significant difference in the Log-Rank test(χ^2=2.057,P=0.151;χ^2=0.452,P=0.501).Cox analysis showed that lymph node metastasis,clinical stage,positive RLIP76 and positive CD44 were independent risk factors affecting the prognosis of gastric cancer(P<0.05).Conclusion The levels of serum RLIP76 and CD44 are abnormally high in patients with

关 键 词：胃癌 RLIP76 CD44 病理 预后 

分 类 号：R735.2[医药卫生—肿瘤]