钛种植体表面Ca-antimiR138复合物修饰促进间充质干细胞成骨分化的研究  

Immobilization of Ca-antimiR138 complex onto titanium implant surface for the enhancement of osteogenic differentiation of mesenchymal stem cells

作　　者：方开秀 侯亚杰 许晓茹 贾森 魏洪波 宋文 FANG Kaixiu;HOU Yajie;XU Xiaoru;JIA Sen;WEI Hong bo;SONG Wen(Department of Prosthodontics,State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi Key Laboratory of Stomatology,,School of Stomatology,The Fourth Military Medical University,Xi’an 710032,China;Department of Implantology,State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi Key Laboratory of Stomatology,School of Stomatology,The Fourth Military Medical University,Xi’an 710032,China;School of Stomatology,Xian Medical University)

机构地区：[1]军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔医学重点实验室,第四军医大学口腔医院修复科,西安710032 [2]军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔医学重点实验室,第四军医大学口腔医院种植科,西安710032 [3]西安医学院口腔医学院

出　　处：《实用口腔医学杂志》2020年第2期284-289,共6页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:31800790);陕西省重点研发计划(编号:2019SF-031);陕西省高校科协青年人才托举计划(编号:20190304)。

摘　　要：目的:利用Ca-antimiR138进行钛种植体表面修饰以提升其表面骨诱导能力。方法:纯钛片抛光后在氢氟酸中进行阳极氧化(20 V,30 min)制备纳米管表面,将Ca-antimiR138复合物吸附于纳米管表面,将间充质干细胞(MSCs)培养在种植体表面并诱导成骨分化,7 d后采用BCIP/NBT试剂盒进行碱性磷酸酶(ALP)染色。结果:Ca-antimiR138复合物可高效吸附在纳米管表面,呈现均匀分布;采用Ca-antimiR138修饰后表面诱导ALP表达提升至10倍以上。结论:应用Ca-antimiR138复合物修饰种植体表面可显著提升其成骨诱导活性。Objective:To immobilize the Ca-antimiR138 complex onto titanium implant surface for the enhancement of osteogenesis(MSCs)of mesenchymal stem cells.Methods:Pure titanium sample surface was polished and anodized in hydrofluoric acid under 20 V for 30 min to formulate the nanotubes(NTs)structure.The Ca-antimiR138 complex was adsorbed and MSCs were seeded onto the NTs surface for the osteogenic differentiation of MSCs.After 7 days induction,the alkaline phosphatase(ALP)activity was determined by BCIP/NBT staining.Results:The Ca-antimiR138 complex was efficiently adsorbed onto NTs surface and showed relative homogeneous distribution.After the antimiR138 was loaded,the ALP activity of MSCs was increased by more than 10 times.Conclusion:The Ca-antimiR138 modified titanium implant surface can improve the osteoinductive ability of titanium implant.

关 键 词：钛种植体 RNA干扰(RNAI) 钙离子 间充质干细胞(MSCs) 成骨分化 

分 类 号：R783.6[医药卫生—口腔医学]