废水处理污泥中邻苯二甲酸酯降解菌的多样性研究  

Study on the diversity of phthalic acid esters degrading microorganisms in activated sludge from wastewater treatment plant

作　　者：王群 方程冉 陈亮 WANG Qun;FANG Chengran;CHEN Liang(Key Laboratory of Recycling and Eco-treatment of Waste Biomass of Zhejiang Province,School of Environmental and Natural Resources,Zhejiang University of Science and Technology,Hangzhou Zhejiang 310023;School of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou Zhejiang 310018)

机构地区：[1]浙江科技学院环境与资源学院,浙江省废弃生物质循环利用与生态处理技术重点实验室,浙江杭州310023 [2]浙江工商大学食品与生物工程学院,浙江杭州310018

出　　处：《环境污染与防治》2020年第4期406-410,共5页Environmental Pollution & Control

基　　金：国家自然科学基金面上项目(No.51678531、No.51878617);国家自然科学基金青年基金资助项目(No.41907276);浙江省自然科学基金青年基金资助项目(No.LQ19D030001)。

摘　　要：系统探究了常规废水处理污泥对典型邻苯二甲酸酯(PAEs)的微生物群落响应特征和污泥中PAEs降解菌的多样性。结果表明:(1)废水处理污泥经过10d培养后,对1000mg/L的邻苯二甲酸二丁酯(DBP)和邻苯二甲酸二异辛酯(DEHP)的降解率分别可达99.9%和83.6%。DEHP的降解速率低于DBP。(2)DBP和DEHP的添加显著降低了废水处理污泥的微生物多样性;DBP、DEHP降解菌富集培养后污泥物种组成相似性较高,且均与废水处理污泥差异较大。(3)废水处理污泥中存在多种类型PAEs降解菌。高浓度DBP和DEHP的存在促使具有DBP/DEHP降解能力的微生物群落不断增长,进而成为优势微生物,最终达到DBP和DEHP的有效降解。The response of microbial community to typical phthalic acid esters(PAEs)and the diversity of PAEs degrading microorganisms in activated sludge from a conventional wastewater treatment plant was systematically explored.The results showed that:(1)after 10 days cultivation of wastewater activated sludge,the degradation rates of di-n-butyl phthalate(DBP)and di-2-ethylhexyl phthalate(DEHP)at a concentration of 1000 mg/L reached 99.9%and 83.6%respectively.And the degradation rate of DEHP was lower than that of DBP.(2)The addition of DBP and DEHP significantly reduced the microbial diversity in the activated sludge.DBP group and DEHP group had higher similarity in microbial community structure after the enrichment culture,both of which were different from the raw activated sludge.(3)PAEs degrading bacteria were widespread in wastewater activated sludge.The existence of high concentration DBP and DEHP made the microorganisms with DBP/DEHP degradation ability grow continuously and then become the dominant genera,and finally the effective degradation of DBP and DEHP were achieved.

关 键 词：邻苯二甲酸酯 邻苯二甲酸二丁酯 邻苯二甲酸二异辛酯 可操作分类单元 微生物群落 

分 类 号：X172[环境科学与工程—环境科学] X703