腺样囊性癌TrkC差异表达的PDX模型的建立与鉴定  

Establishment and Identification of PDX Model for Differentially Expressed of TrkC in Adenoid Cystic Carcinoma

作　　者：马廷耀 王雪莲 陈晓红[1] 许春萍 武骏[1] 江凌燕 许洪波[1] 周晶[1] 宋晓明 李文伟 Ma Tingyao;Wang Xuelian;Chen Xiaohong;Xu Chunping;Wu Jun;Jiang Lingyan;Xu Hongbo;Zhou Jing;Song Xiaoming;Li Wenwei(Contributed equally Department of Otolaryngology,Beijing Tongren Hospital,Capital Medical University,Beijing 100730,China;Yicon(Beijing)Medical Science And Technology Co..Ltd.,Beijing 100716,China)

机构地区：[1]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科,北京100730 [2]亦康(北京)医药科技有限公司,北京100716

出　　处：《肿瘤预防与治疗》2020年第3期208-214,共7页Journal of Cancer Control And Treatment

基　　金：北京市医院管理局临床技术创新项目(编号:XMLX201701)。

摘　　要：目的:建立并鉴定腺样囊性癌(adenoid cystic carcinoma,ACC)关于原肌球蛋白受体激酶C(trypomyosin-receptor kinase C,Trk C)差异表达的人源肿瘤异种移植模型(patient-derived tumor xenograft,PDX)。方法:收集北京同仁医院头颈外科收治的腺样囊性癌患者的新鲜肿瘤组织,皮下接种至免疫缺陷鼠体内,并不断传代至新一批的小鼠体内直至肿瘤稳定生长。分析患者ACC肿瘤组织在免疫缺陷动物体内自然生长状态下的组织病理特征。结果:本次实验共收集ACC标本10例,其中5例成功建立PDX模型,3例完成鉴定。建立的PDX模型病理形态和组织结构与患者肿瘤组织保持一致;3例PDX模型Trk C表达呈现不同的特点。结论:PDX模型可以很好地保留患者的肿瘤异质性,有助于深入研究ACC发病机理,为以Trk C为靶点的临床前药物筛选提供动物模型。Objective:To establish and identify a patient-derived tumor xenograft(PDX)model for the differential expression of TrkC in adenoid cystic carcinoma(ACC).Methods:Fresh tumor tissue of patients with ACC treated in the department of otolaryngology in Beijing Tongren Hospital were collected,subcutaneously inoculated into immunodeficient mice,and continuously passed into a new batch of mice until the tumors stably grew.The histopathological characteristics of ACC tissue in natural state of immunodeficient animals were analyzed.Results:Ten ACC samples were collected in this experiment.PDX models were successfully established for 5 cases.Pathology,morphology and structure of established PDX models were consistent with those of patients'tumor tissue;TrkC expression in the 5 PDX models showed different characteristics.Conclusion:The PDX model can well preserve the tumor heterogeneity,which is conducive to the in-depth study of the pathogenesis of ACC,and provides an animal model for preclinical screening of Trkc-targeted drugs.

关 键 词：腺样囊性癌 人源肿瘤异种移植模型 生长速度 TRKC 

分 类 号：R739.87[医药卫生—肿瘤] R73-35[医药卫生—临床医学]