缺血再灌注损伤对大鼠肝大部切除后残肝组织TNF-α/IL-6/STAT-3信号通路的影响  

Effect of ischemia reperfusion injury on the TNF-α/IL-6/STAT-3 pathway in the remnant liver after subtotal hepatectomy

作　　者：杨会 朱宇麟[2] 王忠慧[1] 陈磊杰[3] YANG Hui;ZHU Yulin;WANG Zhonghui;CHEN Leijie(Department of Anesthesiology,Yunnan Cancer Hospital,Kunming 650000,China;Department of Anesthesiology,The First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China;Orthopedics Department,The Second Affiliated Hospital of Kunming Medical University,Kunming 65000,China)

机构地区：[1]云南省肿瘤医院麻醉科,云南昆明650000 [2]西安交通大学第一附属医院麻醉科,陕西西安710061 [3]昆明医科大学第二附属医院骨科,云南昆明650000

出　　处：《西部医学》2020年第4期510-514,共5页Medical Journal of West China

摘　　要：目的探讨缺血再灌注损伤对肝大部切除后残肝组织TNF-α/IL-6/STAT-3信号通路的影响。方法选取健康、清洁级、雄性SD大鼠60只,体重230~280 g,随机分为单纯肝切除组(SH组)和缺血再灌注组(IR组)。将两组大鼠肝左、中叶切除,残肝(右、尾叶)作不同处理:SH组不阻断其血流,IR组阻断血流30min后恢复灌注。术后1、6、12、24、48h时取各组大鼠残肝组织,测定残肝组织TNF-α、IL-6水平、CyclinD1、Cdk4、STAT-3 mRNA表达及CyclinD1、Cdk4蛋白表达水平。结果与SH组比较,IR组各时点IL-6表达降低,TNF-α于1~12h明显升高,24h、48h时CyclinD1、Cdk4、STAT-3 mRNA及CyclinD1、Cdk4蛋白表达水平降低(均P<0.05)。结论缺血再灌注损伤下调了肝大部切除后残肝组织TNF-α/IL-6/STAT-3信号通路的表达,最终可能抑制了肝细胞的增殖。Objective To investigate the impact of ischemia reperfusion injury on the TNF-α/IL-6/STAT-3 pathway in the remnant liver after subtotal hepatectomy models. Methods 60 male SD rats weighting 230~280 g were obtained and randomly divided into groupⅠ(subtotal hepatectomy, SH), group Ⅱ(ischemia reperfusion, IR). The left and middle liver was resected, and the remnant liver was treated as followed: the blood flow was not blocked in SH group, but blocked 30 minutes in IR group, then reperfused. After 1, 6, 12, 24 and 48 h, respectively, the serum TNF-α, IL-6 was detected. The mRNA of CyclinD1, Cdk4 and STAT-3 were assayed. The protein expression of CyclinD1 and Cdk4 were detected. Results Compared with SH group, the expression of IL-6 declined at each indicated time point, but the content of TNF-α increased in early stage(1~12 h) in IR group. Moreover, the expression of CyclinD1, Cdk4 and STAT-3 was significant decrease in IR group than that in SH group in 24 h and 48 h.Conclusion Ischemia reperfusion injury could reduce the expression of TNF-/il-6/STAT-3 signaling pathway in the residual liver tissue after subtotal hepatectomy, and may ultimately inhibit the proliferation of hepatocytes.

关 键 词：缺血再灌注损伤 肝大部切除 残肝组织 信号通路 

分 类 号：R575[医药卫生—消化系统]