桂枝茯苓丸对大鼠卵巢癌移植瘤的抑瘤作用及其机制  

Anti-tumor effects and mechanism of Guizhi Fuling pill on rat ovarian cancer xenografts

作　　者：邢磊 屈苗苗[2] 张晓云 XING Lei;QU Miaomiao;ZHANG Xiaoyun(Department of Obstetrics and Gynecology,Yanzhou District Affiliated Hospital of Jining Medical College,Yanzhou 272000,Shandong,China;Department of Obstetrics,The Affiliated Hospital of Jining Medical University,Jining 272000,Shandong,China)

机构地区：[1]济宁医学院附属医院兖州院区妇产科,山东兖州272000 [2]济宁医学院附属医院产科,山东济宁272000

出　　处：《西部医学》2020年第4期515-519,共5页Medical Journal of West China

基　　金：山东省自然科学基金资助项目(ZR2014HM108).

摘　　要：目的探讨桂枝茯苓丸对大鼠卵巢癌移植瘤的抑瘤作用及其机制。方法将30只SD雄性大鼠随机分为对照组、模型组和桂枝茯苓丸组,每组10只。模型组和桂枝茯苓丸组皮下接种人卵巢癌HO-8910细胞,建立裸鼠卵巢癌移植瘤模型。造模成功后,桂枝茯苓丸组灌胃桂枝茯苓丸组方,连续灌胃10d,对照组和模型组大鼠灌胃等体积的蒸馏水。治疗后,检测卵巢癌移植瘤的体积和重量,测定大鼠血清卵泡刺激素(FSH)、促黄体生成素(LH)、血清糖链蛋白(CA)125、白介素(IL)-2和IL-6水平,荧光定量PCR和Western Blot检测卵巢癌移植瘤组织中半胱氨酸蛋白酶(Caspase-3)、存活蛋白(survivin)和p21waf/cip蛋白和mRNA的表达。结果与模型组比较,桂枝茯苓丸组卵巢癌移植瘤的体积和重量显著减小,血清IL-2的水平显著升高,血清FSH、LH、CA125、IL-6的水平显著降低,survivin的蛋白和mRNA表达量显著下降,Caspase-3和p21waf/cip的蛋白和mRNA表达量显著升高(均P<0.05)。结论桂枝茯苓丸可以增强机体免疫力,促进肿瘤细胞的凋亡,从而达到抗肿瘤的作用。Objective To explore the anti-tumor effect and mechanism of Guizhi Fuling pill on rat ovarian cancer xenografts. Methods 30 SD male rats were randomly divided into control group, model group and Guizhi Fuling pill group. Model group and Guizhi Fuling pill group were subcutaneously inoculated with human ovarian cancer HO-8910 cells to establish a rat ovarian cancer xenograft model. After successful modeling, Guizhi Fuling pill group was intragastrically administered with Guizhi Fuling pill for 10 d, and control group and model group were given equal volume of distilled water. After treatment, the volume and weight of ovarian cancer xenografts were detected, and the levels of serum follicle stimulating hormone(FSH), luteinizing hormone(LH), serum carbohydrate antigen(CA) 125, interleukin(IL)-2 and IL-6 were measured. Fluorogenic quantitative PCR and Western Blot were used to detect the protein and mRNA expression levels of caspase-3, survivin and p21 waf/cip in ovarian cancer xenografts. Results Compared with model group, the volume and weight of ovarian cancer xenografts were significantly decreased in Guizhi Fuling pill group(P<0.05). The serum IL-2 level was significantly increased(P<0.05). The levels of serum FSH, LH, CA125 and IL-6 were significantly decreased(P<0.05), and the protein and mRNA expression levels of survivin were significantly decreased(P<0.05). The protein and mRNA expression levels of Caspase-3 and p21 waf/cip were significantly increased(P<0.05). Conclusion Guizhi Fuling pill can enhance the immunity of body and promote the apoptosis of tumor cells, thus achieving anti-tumor effects.

关 键 词：桂枝茯苓丸 卵巢癌 凋亡 免疫 

分 类 号：R737.31[医药卫生—肿瘤]