碘-淀粉褪色光度法测定青霉素酶活性  

Determination of penicillinase activity by iodine-starch chromogenic system bleaching photometry

作　　者：戴兴德 张爱菊 张小林 Dai Xing-de;Zhang Ai-ju;Zhang Xiao-lin(Gansu Medical College,Pingliang,744000)

机构地区：[1]甘肃医学院,平凉744000

出　　处：《中国抗生素杂志》2020年第2期157-160,共4页Chinese Journal of Antibiotics

基　　金：甘肃省高等学校创新能力提升项目(No.2019A-159)。

摘　　要：目的 构建褪色光度法测定青霉素酶活性新体系。方法 碘与淀粉显蓝色,青霉素酶催化青霉素水解产物青霉噻唑酸与碘反应,吸光度A变化与青霉素酶活性有关联。结果 在5min内,酶促反应符合一级反应的特征,青霉素酶活性E(U/mL)在0~4U/mL范围内与△A呈良好的线性关系:△A=-0.1612+0.3264E(U/mL),r=0.9965,表观摩尔吸光系数ε=1.88×10~5L/(mol·cm),检测限为0.11U/mL。结论 方法用于自制酶液酶活性测定,结果满意。Objective To establish a new system for the determination of penicillin enzyme activity by bleaching photometry.Methods The quantitative reaction between penicillium thiazolic acid and iodine in iodinestarch chromogenic solution resulted in the decrease of absorbance A.Penicillin enzyme activity can be calculated according to△A.Results The enzymatic reaction was consistent with the characteristics of the first order reaction in 5min,within the range of 0~4U/mL.It showed a good linear relationship between△A and penicillinase activity.The linear regression equation was△A=-0.1612+0.3264E(U/mL),and the linear regression coefficient r was 0.9965.Apparent molar absorption coefficient of penicillin enzymeεwas 1.88×105L/(mol·cm).The limit of detection was 0.11U/mL.Conclusion The method could be used for the determination of penicillin activity in the enzyme solution and the result was satisfactory.

关 键 词：青霉素酶 碘 淀粉 褪色光度法 

分 类 号：R978.1[医药卫生—药品]