小干扰RNA抑制RUNX2对人乳腺癌MCF-7细胞增殖、凋亡与侵袭的影响  

Inhibition of human RUNX2 by small interfering RNA on proliferation,apoptosis and invasion of human breast cancer MCF-7 cells

作　　者：刘壮凯 张瑞山 张鑫丰 李翔 白静慧 徐宏 LIU Zhuang-kai;ZHANG Rui-shan;ZHANG Xin-feng;LI Xiang;BAI Jing-hui;XU Hong(Department of Breast Cancer,Cancer Hospital of China Medical University,Liaoning Cancer Hospital&Institute,Shenyang 110042,China)

机构地区：[1]中国医科大学肿瘤医院辽宁省肿瘤医院乳腺科,辽宁沈阳110042

出　　处：《解剖科学进展》2020年第1期51-53,共3页Progress of Anatomical Sciences

基　　金：辽宁省科技攻关项目(2013225021)。

摘　　要：目的探讨小干扰RNA抑制人RUNT相关转录因子2(RUNX2)对人乳腺癌MCF-7细胞增殖、凋亡与侵袭的影响。方法应用化学合成小干扰RNA技术沉默人乳腺癌MCF-7细胞中RUNX2基因的表达,用逆转录-聚合酶链反应(RT-PCR)和Western blot实验验证基因沉默效率。应用MTT方法检测细胞增殖能力变化,流式细胞仪检测细胞凋亡的变化,Transwell实验检测细胞侵袭能力变化。结果设计的siRNA能够明显抑制人乳腺癌MCF-7细胞RUNX2的表达。RUNX2基因沉默后,人乳腺癌MCF-7细胞的增殖能力降低,细胞凋亡率明显提高,侵袭能力显著降低(P<0.01)。结论RUNX2基因是治疗人乳腺癌MCF-7细胞的潜在靶点。Objective To investigate the effect of small interfering RNA(SIRNA)inhibiting human RUNTrelated transcription factor 2(RUNX2)on proliferation,apoptosis and invasion of human breast cancer MCF-7 cells.Methods The expression of RUNX2 gene in human breast cancer MCF-7 cells was silenced by chemical synthesis of small interfering RNA,the efficiency of gene silencing was verified by RT-PCR and Western blot.MTT assay was used to detect the change of cell proliferation,flow cytometry was used to detect the change of cell apoptosis,and Transwell assay was used to detect the ability of cell invasion.Results The designed siRNA significantly inhibited the expression of RUNX2 in human breast cancer MCF-7 cells.After RUNX2 gene silencing,the proliferation ability of human breast cancer MCF-7 cells was decreased,the apoptotic rate was increased and the invasive ability was decreased significantly(P<0.01).Conclusion RUNX2 gene is a potential target for the treatment of human breast cancer MCF-7 cells.

关 键 词：乳腺癌 MCF-7细胞 RUNT相关转录因子2 增殖 凋亡 侵袭 

分 类 号：R737.9[医药卫生—肿瘤]