肾癌细胞株786-0通过miRNA-30a-5p调节其增殖凋亡及对XIAP表达的影响  

Renal cancer cell line 786-0 regulates proliferation and apoptosis and its effect on XIAP protein expression by miRNA-30a-5p

作　　者：姜拥军 杨清华 李芸 来卫东 武霞 Jiang Yongjun;Yang Qinghua;Li Yun;Lai Weidong;Wu Xia(The Third People's Hospital of Linyi City,Shandong Linyi 276023;Shandong Medical College,Shandong Linyi 276023)

机构地区：[1]临沂市第三人民医院,山东临沂276023 [2]山东医学高等专科学校,山东临沂276023

出　　处：《中国社区医师》2020年第11期5-6,8,共3页Chinese Community Doctors

基　　金：临沂市科技发展计划项目(NO.201919067)。

摘　　要：目的:探讨miRNA-30a-5p在肾癌细胞中的作用及其机制。方法:将miRNA-30a-5p模拟物及抑制物转染至肾癌786-0细胞。检测miRNA-30a-5p在各组细胞中的表达、细胞增殖能力、细胞凋亡情况及凋亡抑制蛋白(XIAP)表达。结果:miRNA-30a-5p mRNA的表达:模拟物组明显上升,未转染组、抑制物组明显下降;细胞增殖能力:各组均有随时间延长而升高的趋势;模拟物组凋亡率高于抑制物组及未转染组,XIAP蛋白的相对表达量低于抑制物组及未转染组。结论:miRNA-30a-5p过表达并通过靶向XIAP抑制肾癌786-0细胞的增殖,促进细胞凋亡。Objective:To investigate the role of miRNA-30a-5p in renal cancer cells and its mechanism.Methods:miRNA-30a-5p mimics and inhibitors were transfected into renal cancer 786-0 cells.The expression of miRNA-30a-5p in each group of cells,cell proliferation ability,apoptosis and XIAP protein expression were detected.Results:The expression of miRNA-30a-5p mRNA was significantly increased in the simulant group,and decreased significantly in the non-transfected group and the inhibitor group.The cell proliferation ability:each group had a tendency to increase with time.The apoptosis rate in the mimic group was higher than that in the inhibitor group and non-transfected group,the relative expression of XIAP protein was lower than that of the inhibitor and non-transfected groups.Conclusion:miRNA-30a-5p is over-expressed and inhibits the proliferation of renal cancer 786-0 cells by targeting XIAP,and promotes apoptosis.

关 键 词：肾癌 miRNA-30a-5p 增殖 凋亡抑制蛋白 

分 类 号：R737.11[医药卫生—肿瘤]