载脂蛋白E基因多态性与2型糖尿病患者发生脑梗死的相关性研究  

Relationship between apolipoprotein E gene polymorphism and cerebral infraction in Chinese type 2 diabetes mellitus patients

作　　者：于雪莹[1] 王娜[2] 李江[1] 马亮[1] 曹永彤[1] Yu Xueying;Wang Na;Li Jiang;Ma Liang;Cao Yongtong(Laboratory Medicine,China-Japan Friendship Hospital,Beijing100029,China;Department of Transfusion Medicine,China-Japan Friendship Hospital,Beijing 100029,China)

机构地区：[1]中日友好医院检验科,北京100029 [2]中日友好医院输血科,北京100029

出　　处：《中华检验医学杂志》2020年第2期160-164,共5页Chinese Journal of Laboratory Medicine

基　　金：国家自然科学基金(81703892)。

摘　　要：目的探讨载脂蛋白E(APOE)基因多态性与2型糖尿病患者发生脑梗死的相关性。方法采用病例-对照的研究方法,收集2016年8月至2019年3月中日友好医院内分泌和神经内科245例2型糖尿病无脑梗死患者(CON组)(男/女,128/117例)和270例2型糖尿病发生脑梗死患者(CI组)(男/女,145/125例),年龄范围40~85岁。采用实时荧光定量PCR技术检测APOE基因的多态性。应用t检验和χ^2检验比较两组间差异。结果CI组有高血压病史的患者(84.12%)显著高于CON组(70.42%)(χ^2=15.91,P<0.05);且CI组患者收缩压(142.78±20.52)mmHg明显高于CON组(133.89±18.58)mmHg(t=-5.16,P<0.05)。CI组与CON组比较,APOEε2/ε3及ε3/ε4基因型频率明显增高,而ε3/ε3基因型频率明显减低(χ^2=11.48,P<0.05);APOE的等位基因频率比较显示,CI组ε4的基因频率高于CON组,而ε3的基因频率低于CON组(χ^2=7.00,P<0.05)。Logistic回归分析显示,高血压史(OR=1.95,P<0.05)、收缩压(OR=1.02,P<0.05)、APOEε2/ε3(OR=2.08,P<0.05)与APOE基因ε3/ε4(OR=1.85,P<0.05)为T2DM患者发生脑梗死的独立危险因素。结论APOE基因的多态性可能与2型糖尿病患者发生脑梗死有关。Objective To investigate the relationship between apolipoprotein E(APOE)gene polymorphism and cerebral infraction(CI)in Chinese type 2 diabetes mellitus(T2DM)patients.Methods This study included 245 samples of T2DM patients without cerebral infraction(CON group)(Male/Female,128/117)and 270 samples of T2DM patients with cerebral infraction(CI group)(Male/Female,145/125)from the department of endocrinology and neurology utilizing real-time fluorescence quantitative PCR technique.The t test andχ^2 test were used to compare the differences between the two groups.Results Patients with a history of hypertension in the CI group(84.12%)were significantly higher than those in the CON group(70.42%)(χ^2=15.91,P<0.05).The systolic blood pressure(142.78±20.52)mmHg of the CI group was significantly higher than that of the CON group(133.89±18.58)mmHg(t=-5.16,P<0.05).Compared with CON group,the frequency of genotypes ofε2/ε3 andε3/ε4 in CI group was significantly higher,while the frequency ofε3/ε3 genotype was significantly lower(χ^2=11.48,P<0.05);the allele frequency of APOEε4 was higher whileε3 was lower in CI group than that in CON group(χ^2=7.00,P<0.05).Logistic regression analysis showed that hypertension history(OR=1.95,P<0.05),high systolic blood pressure(OR=1.02,P<0.05),APOE genotypes ofε2/ε3(OR=2.08,P<0.05)andε3/ε4(OR=1.85,P<0.05)were independent risk factors for cerebral infarction in T2DM patients.Conclusion The polymorphism of APOE gene may be related to cerebral infraction in Chinese T2DM patients.

关 键 词：糖尿病 2型 糖尿病血管病变 脑梗死 载脂蛋白E4 多态现象 遗传 

分 类 号：R74[医药卫生—神经病学与精神病学] R587[医药卫生—临床医学]