Long-term case study of a Wuzhishan miniature pig with diabetes  

作　　者：Miaomiao Niu Yaqian Liu Lei Xiang Yuqiong Zhao Jifang Yuan Yunxiao Jia Xin Dai Hua Chen 

机构地区：[1]Laboratory Animal Center,Chinese PLA General Hospital,Beijing,PR China [2]State Key Laboratory of Kidney Diseases,Chinese PLA General Hospital,Beijing,PR China

出　　处：《Animal Models and Experimental Medicine》2020年第1期22-31,共10页动物模型与实验医学（英文）

基　　金：The work was supported by the National Natural Science Foundation of China under Grant 31472057 and 31802021;Science and Technology Innovation Nursery Foundation of Chinese PLA General Hospital under Grant 16KMM51.

摘　　要：Background:Miniature pigs are attractive animal models for exploring diabetes because they are similar to humans in terms of physiological structure and metabolism.However,little is known about the complications of diabetes in pigs.Methods:In this study,a 28-month observation of a Wuzhishan miniature pig with streptozotocin(STZ)-induced(120 mg/kg)diabetes was conducted,to investigate diabetes-related complications and the possibility of self-recovery in miniature pigs.Blood glucose,serum and urinary biochemistry was measured,and histopathologic examinations of eyes,kidney and pancreas were made.Results:During the observation,diabetic complications of eyes and kidney were observed.The eye complications included bilateral cataracts in the 15th month and degeneration of inner retina and microaneurysm in the 28th month.Kidney complications included glomerular mesangial expansion,focal segmental glomerular sclerosis,and renal tubular epithelial degeneration,but no proteinuria was observed.By 28 months after the application of STZ,with no treatment given,blood glucose had recovered and the number of pancreatic islet beta-cells had increased significantly.Conclusions:We showed that the STZ-induced diabetes model in miniature pigs could accurately mimic the pathological changes of human diabetes,and that pancreatic islet beta-cell regeneration did occur in an adult miniature pig,providing a new means for exploring diabetic complications and pancreatic islet beta-cell regeneration.

关 键 词：BETA-CELL regeneration diabetes-related COMPLICATIONS PIG 

分 类 号：R587.1[医药卫生—内分泌] R-332[医药卫生—内科学]