Age-related rhesus macaque models of COVID-19  

作　　者：Pin Yu Feifei Qi Yanfeng Xu Fengdi Li Peipei Liu Jiayi Liu Linlin Bao Wei Deng Hong Gao Zhiguang Xiang Chong Xiao Qi Lv Shuran Gong Jiangning Liu Zhiqi Song Yajin Qu Jing Xue Qiang Wei Mingya Liu Guanpeng Wang Shunyi Wang Haisheng Yu Xing Liu Baoying Huang Wenling Wang Li Zhao Huijuan Wang Fei Ye Weimin Zhou Wei Zhen Jun Han Guizhen Wu Qi Jin Jianwei Wang Wenjie Tan Chuan Qin 

机构地区：[1]Key Laboratory of Human Disease Comparative Medicine,Chinese Ministry of Health,Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases,Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences and Comparative Medicine Center,Peking Union Medical College,Beijing,China [2]MHC Key Laboratory of Biosafety,National Institute for Viral Disease Control and Prevention,China CDC,Beijing,China [3]Department of Radiology,Bejing Anzhen Hospital,Capital Medical University,Beijing,China [4]Institute of Pathogen Biology,Chinese Academy of Medical Sciences,Beijing,China [5]Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China

出　　处：《Animal Models and Experimental Medicine》2020年第1期93-97,共5页动物模型与实验医学（英文）

基　　金：This work was supported by the National Research and Development Project of China(Grant No.2020YFC0841100);Fundamental Research Funds for CAMS of China(Grant No.2020HY320001);National Key Research and Development Project of China(Grant No.2016YFD0500304);CAMS initiative for Innovative Medicine of China(Grant No.2016-I2M-2-006);National Mega projects of China for Major Infectious Diseases(Grant No.2017ZX10304402);and National Key Research and Development Programme of China(2016YFD0500301,2020YFC0840800,2020YFC0840900).

摘　　要：Background:Since December 2019,an outbreak of the Corona Virus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus(SARS-CoV-2)in Wuhan,China,has become a public health emergency of international concern.The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models.Methods:Three 3-5 years old and two 15 years old rhesus macaques were intratracheally infected with SARS-CoV-2,and then analyzed by clinical signs,viral replication,chest X-ray,histopathological changes and immune response.Results:Viral replication of nasopharyngeal swabs,anal swabs and lung in old monkeys was more active than that in young monkeys for 14 days after SARS-CoV-2 challenge.Monkeys developed typical interstitial pneumonia characterized by thickened alveolar septum accompanied with inflammation and edema,notably,old monkeys exhibited diffuse severe interstitial pneumonia.Viral antigens were detected mainly in alveolar epithelial cells and macrophages.Conclusion:SARS-CoV-2 caused more severe interstitial pneumonia in old monkeys than that in young monkeys.Rhesus macaque models infected with SARS-CoV-2 provided insight into the pathogenic mechanism and facilitated the development of vaccines and therapeutics against SARS-CoV-2 infection.

关 键 词：PATHOGENICITY PNEUMONIA RHESUS MACAQUE model SARS-CoV-2 

分 类 号：R563.1[医药卫生—呼吸系统] R-332[医药卫生—内科学]