应用中断时间序列分析评价高通量测序技术对临床病毒学的影响  

Application of interruption-time series analysis to evaluate the impact of high-throughput sequencing on clinical virology

作　　者：李洋[1] 张益[1] 于石成[2] 何小周[1] 杨梦婕[1] 王佶[1] 金承刚[3] 马学军[1,4] 王琦琦 Li Yang;Zhang Yi;Yu Shicheng;He Xiaozhou;Yang Mengjiie;Wang Ji;Jin Chenggang;Ma Xuejun;Wang Qiqi(NHC Key Laboratory of Medical Virology and Viral Diseases,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Office of Epidemiology,Chinese Center for Disease Control and Prevention,Beijing 102206,China;School of Social Development and Public Policy,Beijing Normal University,Beijing 100875,China;Center for Biosafety Mega-Science,Chinese Academy of Sciences,Wuhan 430071,China)

机构地区：[1]国家卫生健康委员会医学病毒和病毒病重点实验室,中国疾病预防控制中心病毒病预防控制所,北京102206 [2]中国疾病预防控制中心流行病学办公室,北京102206 [3]北京师范大学社会发展与公共政策学院,100875 [4]中国科学院生物安全大科学中心,武汉430071

出　　处：《中华实验和临床病毒学杂志》2020年第1期23-28,共6页Chinese Journal of Experimental and Clinical Virology

基　　金：国家传染病重大专项(2018ZX10711001,2017ZX10302301-004-002,2017ZX10104001,2018ZX10713-002);国家重点研发计划(2018YFC1315300,2018YFC1315305)。

摘　　要：目的评估全球范围内大规模高通量测序技术的应用对临床病毒学的定量影响。方法分别收集美国国立生物技术信息中心(National Center for Biotechnology Information)2000-2018年如下记录:每年新增全基因组病毒记录数量;每年新增病毒发现文章数量;每年新增病毒准种文章数量;每年新增病毒感染基因标志物文章数量。然后应用中断时间序列法分析上述记录的变化趋势。结果自2008年全球测序中心开始从Sanger法转向使用高通量测序技术以来,各记录每年新增数量分别是使用之前的3.755倍,2.760倍,6.195倍以及3.885倍。各记录长期变化趋势如下:全基因组病毒记录数量每年新增1639.991条(P<0.001);病毒发现文章数量每年新增83.091篇(P<0.001);病毒准种文章数量每年新增2.509篇(P<0.001);病毒感染基因标志物基因感染签名文章每年新增30.836篇(P<0.001)。结论2008年全球测序中心开始应用高通量测序技术,测序成本不断降低,使得高通量测序技术在临床病毒学的应用中日益丰富,对临床病毒学的发展产生了积极深远的影响。Objective To assess the quantitative impact of the application of high-throughput sequencing worldwide on clinical virology.Methods The following records from 2000 to 2018 were collected from National Center for Biotechnology Information:The number of complete genome of virus records per year;the number of virus discovery articles per year;the number of virus quasi-species articles per year;the number of viral infection gene signature articles per year.The method of interrupted time series was applied to analyze the trends of those records.Results Since the sequencing centers worldwide began to transit from the Sanger sequencing to the high-throughput sequencing technology in 2008,the annual value of records mentioned above increased by 3.755,2.760,6.195 and 3.885 times respectively.The long-term trends of change of each record are as follows:the number of complete genome of virus records increased by 1639.991 per year(P<0.001);the number of virus discovery articles increased by 83.091 per year(P<0.001);the number of virus quasi-species articles increased by 2.509 per year(P<0.001);the number of viral infection gene signature articles increased by 30.836 per year(P<0.001).Conclusions In 2008,the sequencing centers worldwide began to apply high-throughput sequencing technology which led to continuous decrease of the cost for sequencing.It result ed in not only increasingly enriched applications in clinical virology but also a long-term positive impact on the advance of clinical virology.

关 键 词：中断时间序列设计 项目评价 高通量测序 临床病毒学 

分 类 号：R3[医药卫生—基础医学]