孟加拉国输入芜湖2型登革病毒E基因分子特征  

Molecular characterization of envelope gene of dengue virus type 2 imported from Bangladesh to Wuhu, Anhui provice, China

作　　者：王共飞[1] 安洲[1] 程周祥[1] 史永林[2] 苏斌[2] Wang Gongfei;An Zhou;Cheng Zhouxiang;Shi Yongling;Su Bin(Wuhu Center for Disease Control and Prevention,Wuhu 241000,China;Anhui Provincial Center for Disease Control and Prevention,Hefei 230601,China)

机构地区：[1]芜湖市疾病预防控制中心,241000 [2]安徽省疾病预防控制中心,合肥230601

出　　处：《中华实验和临床病毒学杂志》2020年第1期33-37,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：安徽省十三五传染病科研专项(2018ZX10713003-002-005)。

摘　　要：目的确定1例由孟加拉国输入芜湖的登革热病例及登革病毒(dengue virus,DENV)的E基因分子特征。方法提取患者血清标本核酸,使用Real-time RT-PCR方法进行DENV核酸通用及1/2/3/4血清型筛查。测定E基因完整序列,采用MEGA7.0软件构建E基因系统进化树,并进行E基因核苷酸和氨基酸序列比对分析。结果患者血清呈现2型DENV核酸阳性。E基因序列系统进化分析显示属于Cosmopolitan基因型,与2014年马来西亚输入中国台湾分离株(D2/Malaysia/1408aTw)同源性最高,核苷酸同源性为99.60%,氨基酸同源性为99.80%,有6处核苷酸差异(42:G→A;141:G→A;490:G→A;516:A→G;954:A→G;1344:C→T),1处氨基酸差异(164:V→I)。E蛋白拥有弱毒株特征位点E126-E和强毒株特征位点E383~385-E-P-G。结论输入型患者为2型DENV感染且该病毒起源于马来西亚。Objective To determine the genotype of dengue virus(DENV)and its molecular characteristics of E gene from 1 case imported from Bangladesh to Wuhu city.Methods The viral RNA was extracted from patient serum sample,the serotype of DENV was determined by Real-time RT-PCR through screening the universal and serotypes 1/2/3/4 of DENV.The completed sequence of DENV E gene was amplified,the phylogenetic tree of E gene was constructed by MEGA 7.0 software,and the nucleotide and amino acid sequences alignments of E gene were analyzed.Results Patient serum was positive for DENV type 2 nucleic acid.The DENV type 2 belonged to the Cosmopolitan genotype by phylogenetic analysis of the E gene,and it had the highest homology with the isolate D2/Malaysia/1408aTw which was imported from Malaysia to Taiwan in 2014,the homologies of nucleotide and amino acid reached 99.60%and 99.80%,respectively.Totally 6 nucleotides(42:G→A;141:G→A;490:G→A;516:A→G;954:A→G;1344:C→T)and 1 amino acid(164:V→I)had changed in E gene.E protein possessed attenuated strain characteristic site E126-E and virulent strain characteristic site E383-385-E-P-G.Conclusions The imported patient is infected with DENV type 2 and the virus is originated from Malaysia.

关 键 词：登革病毒 E基因 系统进化分析 

分 类 号：R51[医药卫生—内科学]