添加环格列酮对延边黄牛骨骼肌卫星细胞成脂转分化的影响  

Effect of Ciglitazone on Adipogenic Transdifferentiation of Skeletal Muscle Satellite Cells in Yanbian Yellow Cattle

作　　者：闫研 张军芳 孙斌 王英 孙建富 孙晓蛟 崔岩 金鑫 严昌国 李香子 YAN Yan;ZHANG Junfang;SUN Bin;WANG Ying;SUN Jianfu;SUN Xiaojiao;CUI Yan;JIN Xin;YAN Changguo;LI Xiangzi(Co-Innovation Center of Beef Cattle Science and Industry Technology,Yanbian University,Yanji 133000,China)

机构地区：[1]延边大学,肉牛科学与产业技术协同创新中心,延吉133000

出　　处：《中国畜牧兽医》2020年第3期714-721,共8页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然基金资助项目(21660667)。

摘　　要：试验旨在探究过氧化物酶体增殖物激活受体(PPARγ)激动剂(环格列酮)对延边黄牛骨骼肌卫星细胞成脂转分化的影响。试验分为4组:对照组(CON,不添加环格列酮)、CL组(5μmol/L环格列酮)、CM组(10μmol/L环格列酮)和CH组(20μmol/L环格列酮),利用不同浓度环格列酮处理骨骼肌卫星细胞96 h后,通过油红O染色法和甘油三酯的测定来验证脂滴的形成,并使用实时荧光定量PCR和Western blotting测定基因表达和蛋白质定量的变化来验证脂肪细胞的生成。甘油三酯测定和油红O染色显示,与对照组相比,添加环格列酮后,骨骼肌卫星细胞内有脂滴生成,且脂滴形成量和甘油积累量与环格列酮添加量呈正相关。实时荧光定量PCR和Western blotting结果表明,与对照组相比,环格列酮组显著增加了成脂转录因子PPARγ、CCAAT/增强子结合蛋白α(C/EBPα)和围脂滴蛋白-2(PLIN2)基因的表达,显著下调了成肌相关因子MyoD的表达(P<0.05),且所有蛋白与基因表达趋势保持一致,表明环格列酮可以促进牛骨骼肌卫星细胞向脂肪细胞分化。This study was aimed to investigate the effect of ciglitazone,a peroxisome proliferator-activated receptor(PPARγ)agonist,on the adipogenic transdifferentiation of skeletal muscle satellite cells in Yanbian Yellow cattle.The skeletal muscle satellite cells were treated with ciglitazone at concentrations of 0(CON group),5(CL group),10(CM group)and 20(CH group)μmol/L for 96 h.The formation of lipid droplets was evaluated by oil red O staining and triglyceride amounts was determined by triglyceride determination kit.Real-time quantitative PCR and Western blotting were employed to detect whether adipocytogenesis related factors were involved.The results showed that the area of oil red O and the amounts of triglyceride were both significantly increased after treated with ciglitazone compared with control group(P<0.05).Moreover,the area of oil red O and the amounts of triglyceride were positively correlated with the concentration of ciglitazone.Compared with control group,the mRNA and protein expression of adipocytic transcription factors,including PPARγ,CCAAT/enhancer binding protein alpha(C/EBPα)and peripoietin-2(PLIN2),were significantly upregulated in ciglitazone addition groups(P<0.05).However,the expression of myogenic-related factor MyoD was downregulated after treated with ciglitazone(P<0.05).The results indicated that ciglitazone could promote the transdifferentiation of skeletal muscle satellite cells to adipocytes in cattle.

关 键 词：延边黄牛 骨骼肌卫星细胞 脂肪生成 过氧化物酶体增殖物激活受体(PPARγ) 环格列酮 

分 类 号：Q952.6[生物学—动物学]