青砖茶水提物对HepG2细胞脂肪变性的干预作用  

Intervention Effect of Water Extract of Green Brick Tea on Steatosis of HepG2 Cells

作　　者：余婕 闫梦真 陈桂婷 周婷婷 李世刚[1,2] YU Jie;YAN Mengzhen;CHEN Guiting;ZHOU Tingting;LI Shigang(School of Medicine,China Three Gorges Univ.,Yichang 443002,China;The Research Institute of Hubei Province Chang-Sheng-Chuan Green Brick Tea,Yichang 443000,China)

机构地区：[1]三峡大学医学院,湖北宜昌443002 [2]湖北省长盛川青砖茶研究所,湖北宜昌443000

出　　处：《三峡大学学报（自然科学版）》2020年第2期107-112,共6页Journal of China Three Gorges University：Natural Sciences

基　　金：国家自然科学基金资助项目(81274166,81673665)。

摘　　要：为了探讨青砖茶水提物(green brick tea water extract,GBT-WE)对胎牛血清诱导的HepG2细胞脂肪变性的干预作用及其机制,利用高浓度胎牛血清(fetal bovine serum,FBS)的培养基对HepG2细胞培养48h,建立非酒精性细胞脂肪变性模型,而后加入不同质量浓度GBT-WE给药处理24h.采用CCK-8法检测含有不同质量浓度FBS的培养液和GBT-WE对细胞增殖的影响,通过油红O染色观察细胞脂肪变性的程度,利用酶法检测甘油三酯(triglyceride,TG)和总胆固醇(total cholesterol,TC)的含量变化,采用Western blot检测脂质代谢相关蛋白固醇调节元件结合蛋白1c(Sterol-regulatory element binding proteins 1c,SREBP1c)、脂肪酸合成酶(Fatty acid synthase,FAS)的表达水平.结果显示:含有50%FBS的培养基培养HepG2细胞48h可诱导其脂肪变性;GBT-WE的质量浓度超过240μg/mL时对HepG2细胞的增殖有明显的抑制作用;GBTWE能显著减轻细胞脂肪变性的程度(P<0.01),显著降低TG、TC表达量(P<0.01),同时可以下调SREBP1c、FAS蛋白表达水平(P<0.01).上述实验结果初步表明青砖茶水提物通过SREBP1c/FAS信号通路实现对HepG2细胞脂肪变性的改善作用.In order to investigate the protective effect and mechanism of water extract of Green brick tea(GBT-WE)on the steatosis of HepG2 cells induced by fetal bovine serum.HepG2 cells were cultured for 48h in a medium with high concentrations of fetal bovine serum to establish a nonalcoholic cell steatosis model.Then,different concentrations of GBT-WE were added for treatment for 24 hours.The effects of different concentrations of FBS medium and GBT-WE on cell proliferation were detected by CCK-8.Oil red O staining was used to observe the degree of cell steatosis.The content of triglyceride(TG)and total cholesterol(TC)was detected by Enzymatic method.The expression of Sterol-regulatory element binding protein 1c and fatty acid synthase were detected by Western blot.The results showed that HepG2 cells were cultured in 50%FBS for 48h to induce steatosis.When the concentration of GBT-WE exceeded 240μg/mL,the proliferation of HepG2 cells was significantly inhibited.GBT-WE significantly reduced the degree of cell steatosis(P<0.01),and significantly decreased the expression of TG and TC(P<0.01).At the same time,the expression levels of SREBP1c and FAS protein were down-regulated(P<0.01).The above experimental results preliminarily showed that the water extract of green brick tea can improve the steatosis of HepG2 cells through the SREBP1c/FAS signaling pathway.

关 键 词：青砖茶水提物(GBT-WE) HEPG2细胞 非酒精性脂肪肝(NAFLD) 脂肪变性 从头脂肪生成(DNL) 

分 类 号：R575.5[医药卫生—消化系统]