蛴螬提取物对兔光损伤视网膜变性Bax、Caspase-3表达的影响  

Effects of Different Doses of Grub Extract on Expressions of Bax and Caspase-3 in Rabbits with Light Damage

作　　者：蒋鹏飞[1,2] 吴大力 彭俊[2,4] 彭清华 JIANG Pengfei;WU Dali;PENG Jun;PENG Qinghua(Hunan University of Chinese Medicine,Changsha 410208,Hunan,China;Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Eye,Ear,Nose and Throat Diseases in Hunan Province,Changsha 410208,Hunan,China;The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine,Nanning 530023,Guangxi,China;The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,Hunan,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]中医药防治眼耳鼻咽喉疾病湖南省重点实验室,湖南长沙410208 [3]广西中医药大学第一附属医院,广西南宁530023 [4]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《辽宁中医杂志》2020年第2期178-180,I0002,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(30772824,81574031);湖南省中医药科研基金(2010012);湖南省教育厅科研基金(09C729);湖南中医药大学科研基金(3601010157);中医药防治五官科疾病湖南省重点实验室建设项目(2017TP1018);中央财政支持地方高校建设项目;国家中医药管理局中医眼科学重点学科建设项目;湖南省中医五官科学重点学科建设项目。

摘　　要：目的探讨蛴螬提取物对兔光损伤视网膜变性Bax、Caspase-3表达的影响。方法将30只实验性兔随机分成5组,分别为空白组、模型组、蛴螬低剂量组、蛴螬中剂量组、蛴螬高剂量组,每组都是6只动物,12只眼。除空白组外,其余4组均建立光损伤视网膜变性模型,在给药14 d处死全部兔子后取材做Bax、Caspase-3免疫组化检测,计算机系统图象分析结果并进行统计分析。结果与模型对照组比较,蛴螬中、高剂量治疗组的Bax、Caspase-3积分光密度增加,差异具有统计学意义(P<0.05)。结论蛴螬通过增加Bax、Caspase-3的表达,抑制细胞凋亡受体信号通路来发挥保护视网膜作用,中、高剂量组效果较好。Objective To investigate the effects of grub extract on the expressions of Bax and Caspase-3 in rabbits with photorefractive degeneration.Methods Thirty experimental rabbits were randomly divided into 5 groups:blank group,model group,grublow,middle and high dose groups,each group consisting of 6 animals and 12 eyes.Except the blank group,the other four groups established a model of light loss retinal degeneration.All rabbits were sacrificed after 14 days administration,and expressions of Bax and Caspase-3 were tested by immunohistochemistry method.The computer system image analysis results were analyzed statistically.Results Compared with the model control group,the Bax and Caspase-3 integral optical density of the middle and high dose groups increased,and the difference was significant(P<0.05).Conclusion By increasing the expressions of Bax and Caspase-3 and inhibiting the apoptotic death receptor signaling pathway,the grub middle and high dose groups performed better.

关 键 词：蛴螬提取物 光损伤 BAX CASPASE-3 

分 类 号：R774.13[医药卫生—眼科]