CDH23基因多态性与噪声性听力损失发生风险关系研究  

Relationship research among CDH23 gene and the risk of noise-induced hearing loss

作　　者：焦洁 谷桂珍 陈国顺 张焕玲 吴辉 李艳红 周文慧 余善法 Jiao Jie;Gu Guizhen;Chen Guoshun;Zhang Huanling;Wu Hui;Li Yanhong;Zhou Wenhui;Yu Shanfa(Henan Provincial Institute for Occupational Health,Zhengzhou 450052,China;Wugang Institute for Occupational Health,Wugang 462500,China;Henan Medical College,Zhengzhou 451191,China)

机构地区：[1]河南省职业病防治研究院,郑州450052 [2]舞钢劳动卫生职业病防治研究所,462500 [3]河南医学高等专科学校,郑州451191

出　　处：《中华劳动卫生职业病杂志》2020年第2期84-90,共7页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金项目(81372940、81872574);国家科技支撑计划项目(2014BAI12B03)。

摘　　要：目的探讨CDH23基因多态性与噪声性听力损失(noise-induced hearing loss,NIHL)发生风险的关系.方法从2006年1月1日起,以河南省某钢铁企业的6297名接触噪声作业工人为队列研究人群,随访至2015年12月31日.于2019年7月,以1∶1巢式病例对照研究方法在队列研究对象中按照年龄、接噪工龄、性别、工种因素选择双耳高频(3000、4000、6000 Hz)平均听阈≥40 dB者选为听力损失组,选择双耳高频平均听阈<35 dB任一耳语频的任一频段(500、1000、2000 Hz)听阈均≤25 dB者为对照组,两组研究对象各286例.对调查对象进行一般体格检查和问卷调查,进行纯音听力测试和作业现场噪声测量,采用中高通量单核苷酸多态性分型检测技术(SNPscanTM法)对研究对象的CDH23基因18个位点进行检测.并采用条件Logistic回归分析不同单核苷酸多态性(single nucleotide polymorphism,SNP)与NIHL的关系,及调整协变量后不同多态位点与NIHL发生风险的关系;以不同累积噪声暴露量(cumulative noise exposure,CNE)分层后,采用条件logistic回归对不同位点与NIHL发生风险关系进行分析.采用广义多因子降维法(generalized multifactor dimensionality reduction,GMDR)分析不同SNP组合与NIHL发生风险的关系.结果与对照组比较,听力损失组在年龄、接噪工龄、CNE、饮酒习惯、高血压患病情况和体育锻炼情况的分布差异均无统计学意义(P>0.05);听力损失组研究对象吸烟人数多,听力损失组双耳高频平均听阈位移高,差异有统计学意义(P<0.0l).在调整了CNE、吸烟、饮酒、体育锻炼、高血压混杂因素的条件下,分析结果显示CDH23基因rs3802711、rs3752751、rs3752752、rs11592462、rs10762480、rs3747867多态位点在总体或CNE分层分析结果与NIHL发生风险均有关(P<0.05).rs3802711位点总体分析结果和CNE≥97 dB(A)·年分层分析结果均显示,与携带GG基因型的噪声作业工人比较,携带AA/GA或GA+AA基因型的噪声�Objective To explore the relationship among CDH23 gene variation and the risk of noise-induced hearing loss(NIHL).Methods The nested case-control study was performed and this study followed a cohort of 6297 noise-exposed workers in a steel factory of Henan province in China from January 1,2006 to December 31,2015.In July 2019,subjects whose average hearing threshold were more than 40 dB in high frequency were defined as the case group,and subjects whose average hearing threshold were less than 35 dB in high frequency and less than 25 dB in speech frequency were defined as the control group.A nested case-control study which included 572 subjects was carried out,in which subjects consisted of 286 cases and 286 controls.18 single nucleotide polymorphisms(SNPs)in CDH23 were selected and genotyped,then we analyzed the association among SNPs in CDH23,haplotypes in CDH23 and NIHL risk.Logistic regression was performed to analyze the main effects of SNPs and the interactions between CNE and SNPs adjusting cumulative noise exposure(CNE),smoking,drinking,physical exercise and hypertension.Moreover,the association between haplotypes in CDH23 and NIHL risk were also analyzed.We ananlyzed the relationship amongst different SNP groups and NIHL risk using the generalized multifactor dimensionality reduction(GMDR)method.Results The results suggested that significant associations were observed for rs3802711,rs3752751,rs3752752,rs11592462,rs10762480,rs3747867 for NIHL overall and/or various CNE strata by adjusting CNE,smoking,drinking,physical exercise and blood pressure.For rs3802711,workers exposure to noise carrying the AA/GA genotype of rs3802711 increased risk of NIHL than those carrying GG genotype(OR=3.121;95%CI:1.054-9.239,P=0.035)in overall;In the stratified analysis of CNE(>97 dB(A)·year at rs3802711 locus,workers exposure to noise carrying GA genotype(OR=2.056;95%CI:1.226~3.448,P=0.006)and GA+AA/GA genotype(OR=2.221;95%CI:1.340~3.681,P=0.002)increased NIHL risk.For rs11592462,workers exposure to noise carrying the GG g

关 键 词：噪声性听力损失 单核苷酸多态性 CDH23基因 基因多态性 累积噪声暴露量 

分 类 号：R[医药卫生]