肉碱对冻融猪精子总抗氧化能力、抗氧化酶基因及凋亡相关基因表达的影响  

Effects of Carnitine on Total Antioxidant Capacity and Expression of Antioxidant Enzyme and Apoptosis Related Genes of Frozen-thawed Boar Sperm

作　　者：吕艳秋 徐曼 徐一超 程咪咪 金一[1] LYU Yanqiu;XU Man;XU Yichao;CHENG Mimi;JIN Yi(Yanbian University,Yanji 133002,China)

机构地区：[1]延边大学,延吉133002

出　　处：《中国畜牧兽医》2020年第4期1121-1129,共9页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31960659)。

摘　　要：试验旨在探究肉碱对冻融猪精子质量、抗氧化、抗凋亡及精卵结合能力的影响。试验分鲜精组和冻融组,冻融组的肉碱浓度分别为0、0.025、0.05、0.075 mg/mL,对冻融后的精子质量、总抗氧化能力、抗氧化酶相关基因及凋亡相关基因mRNA表达、精卵结合能力进行检测。结果显示,在冻融组中,经过肉碱处理的精子存活率、质膜完整率和顶体膜完整率均显著高于0 mg/mL处理组(P<0.05),其中肉碱浓度为0.05 mg/mL时改善效果最好;经过冻融处理的精子中丙二醛(MDA)浓度与鲜精组相比显著升高(P<0.05),其中肉碱浓度为0.05 mg/mL时显著低于其他肉碱处理组(P<0.05);与鲜精组相比,各冻融组精子总抗氧化能力均显著降低(P<0.05),肉碱浓度为0.05 mg/mL时总抗氧化能力最高。此外,与鲜精组相比,0 mg/mL肉碱处理组中凋亡相关基因Caspase-3与Bax的相对表达量显著升高(P<0.05),抗凋亡基因Bcl-2的相对表达量显著降低(P<0.05),抗氧化酶相关基因SOD 2、CAT与GPx的相对表达量显著降低(P<0.05);冻融组精卵结合能力均下降,但肉碱浓度为0.05 mg/mL时可得到显著改善(P<0.05)。结果表明,添加肉碱可以改善冻融猪精子的质量、抗氧化能力、抗凋亡能力及精卵结合能力。This study was aimed to investigate the effects of carnitine on frozen-thawed boar sperm quality,anti-oxidation,anti-apoptosis and sperm-egg fertilization ability in vitro.The sperm was divided into a fresh sperm group and 4 frozen-thawed sperm groups.The frozen-thawed sperm groups were treated with different concentrations of carnitine(0,0.025,0.05 and 0.075 mg/mL).The quality,total antioxidant capacity,sperm-egg binding ability,mRNA expression of antioxidant enzyme related and apoptosis-related genes of sperm were investigated.The results showed that in frozen-thawed groups,carnitine treatment significantly increased sperm motility,plasma membrane integrity rate and acrosome membrane integrity rate compared with 0 mg/mL treatment(P<0.05),meanwhile treatment with 0.05 mg/mL carnitine showed the highest improvement.The concentration of MDA in sperm was significantly increased after frozen-thaw treatment(P<0.05),among them,a significant decrease was only measured in 0.05 mg/mL treatment group.Compared with fresh sperm group,the total antioxidant capacity of freeze-thaw group sperm decreased significantly(P<0.05),0.05 mg/mL carnitine treatment group showed the highest total antioxidant capacity in frozen-thawed groups.Furthermore,compared with fresh sperm group,the relative expression of apoptosis-related genes Caspase-3 and Bax increased significantly(P<0.05),the relative expression of anti-apoptotic gene Bcl-2 decreased significantly(P<0.05),and the expression of antioxidant enzyme-related genes SOD2,CAT and GPx decreased significantly in 0 mg/mL carnitine treatment(P<0.05).Compared with fresh sperm group,the ability of sperm-egg binding in every treatment group showed a decrease,0.05 mg/mL carnitine treatment also showed the highest improvement(P<0.05).The results indicated that the addition of carnitine could improve the quality,anti-oxidation ability,anti-apoptosis ability and sperm-egg binding ability of frozen-thawed boar sperm.

关 键 词：肉碱 猪精子 冻存保存 抗凋亡 抗氧化酶 

分 类 号：S814.8[农业科学—畜牧学]