苦参碱眼液调控TLR4/NF-κB通路影响实验性葡萄膜炎家兔相关细胞因子的表达  

Effects of matrine eye drops on levels of cytokines associated with experimental uveitis in rabbits by regulating TLR4/NF-κB pathway

作　　者：邓珍[1] 吴一峰[1] 姜蕾[1] 唐丹燕[1] 俞国孝 姚莉 吴坤林[1] DENG Zhen;WU Yi-feng;JIANG Lei;TANG Dan-yan;YU Guo-xiao;YAO Li;WU Kun-lin(Department of Ophthalmology,the First Peoples Hospital of Yuhang District,Hangzhou,Zhejiang 311100,China)

机构地区：[1]杭州市余杭区第一人民医院眼科,浙江杭州311100

出　　处：《中华全科医学》2020年第4期568-572,669,共6页Chinese Journal of General Practice

基　　金：浙江省医药卫生科技计划项目(2017KY238);杭州市余杭区科学技术局项目(2015011)。

摘　　要：目的探究苦参碱眼液调控Toll样受体4/核因子-κB(TLR4/NF-κB)通路对家兔实验性葡萄膜炎相关细胞因子水平的影响。方法建立实验性葡萄膜炎家兔模型,采用随机数字表的方法分为模型组、妥布霉素地塞米松滴眼液组、苦参碱眼液低(0.50 g/L)、中(0.75 g/L)、高(1.00 g/L)剂量组,每组8只,另取8只设为对照组。造模后妥布霉素地塞米松滴眼液组、苦参碱眼液低、中、高剂量组分别以妥布霉素地塞米松滴眼液、苦参碱眼液滴眼治疗,对照组、模型组家兔以相同剂量生理盐水滴眼,持续7 d。裂隙灯观察各组家兔眼前节炎症反应并对其进行临床评分,苏木精-伊红染色(HE)观察各组家兔视网膜组织病理形态并对其进行病理评分,酶联免疫吸附法(ELISA)检测各组家兔血清及房水中细胞因子[肿瘤坏死因子α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-1(IL-1)]水平,蛋白免疫印迹法(Western blotting)检测视网膜组织中TLR4/NF-κB通路蛋白TLR4、MyD88、NF-κB p65的表达。结果与对照组相比,模型组家兔临床评分、病理评分,血清和房水中IL-6、IL-1、TNF-α水平,视网膜组织中TLR4、MyD88、NF-κB p65表达均显著升高(均P<0.05);与模型组相比,苦参碱眼液各剂量组和妥布霉素地塞米松滴眼液组家兔临床评分、病理评分,血清和房水中IL-6、IL-1、TNF-α水平,视网膜组织中TLR4、MyD88、NF-κB p65表达均降低且苦参碱眼液各剂量组之间呈剂量依赖性(均P<0.05);苦参碱眼液高剂量组和妥布霉素地塞米松滴眼液组各指标相比,差异无统计学意义(P>0.05)。结论苦参碱眼液可降低血清及房水中细胞炎症因子水平,减轻炎症反应,改善家兔葡萄膜炎症状,可能是通过下调TLR4/NF-κB通路实现。Objective To investigate the effect of matrine eye drops on the levels of cytokines associated with experimental uveitis in rabbits by regulating toll like receptor 4/nuclear factor-κB(TLR4/NF-κB) pathway. Methods The rabbit model of experimental uveitis was established and randomly divided into model group, tobramycin-dexamethasone eye drops group, low(0.50 g/L), medium(0.75 g/L) and high(1.00 g/L) dose matrine eye drops groups, with 8 rabbits in each group. Another 8 rabbits were selected as control group. After successfully modeling, tobramycin-dexamethasone eye drops group, low, medium and high dose matrine eye drops groups were treated with tobramycin-dexamethasone eye drops and matrine eye drops respectively, rabbits in all groups were given the same dose of saline eye drops for 7 days. Slit lamp was used to observe and evaluate the inflammation of anterior segment of rabbit eyes. Hematoxylin-eosin staining(HE) was used to observe the pathological morphology of the retina of rabbits. Enzyme-linked immunosorbent assay(ELISA) was used the levels of cytokines, tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and interleukin-1(IL-1) in serum and aqueous humor of rabbits, and Western blot was used to detect the expressions of TLR4/NF-κB pathway proteins TLR4, MyD88 and NF-κB p65 in retina. Results Compared with the control group, the clinical and pathological scores, the levels of IL-6, IL-1 and TNF-α in serum and aqueous humor, and the expressions of TLR4, MyD88 and NF-κB p65 in retina were significantly increased in the model group(all P<0.05). Compared with model group, the clinical and pathological scores, the levels of IL-6, IL-1 and TNF-α in serum and aqueous humor, and the expressions of TLR4, MyD88 and NF-κB p65 in retina tissue in all dose matrine eye drops groups and tobramycin-dexamethasone eye drops group were decreased, and there was a dose-dependent relationship among the dose groups of matrine eye drops(all P<0.05). There was no significant difference between the high dose matrine eye

关 键 词：苦参碱眼液 家兔 葡萄膜炎 TLR4/NF-κB通路 细胞因子 

分 类 号：R773[医药卫生—眼科] R988.1[医药卫生—临床医学]