青藏高原冰川棘豆适应性分子机制的转录组学初步研究  

Preliminary study on transcriptomics of adaptive molecular mechanism of Oxytropis glacialis in the Qinghai-Tibet Plateau

作　　者：姬亚丽 陈飞飞 许国琪 曹鹏熙 李敬科 刘星[1,2] JI Ya-li;CHEN Fei-fei;XU Guo-qi;CAO Peng-xi;LI Jing-ke;LIU Xing(College of Science,Tibet University,Lhasa 850000,China;College of Life Science,Wuhan University,Wuhan 430072,China)

机构地区：[1]西藏大学理学院,西藏拉萨850000 [2]武汉大学生命科学学院,湖北武汉430072

出　　处：《环境生态学》2020年第4期39-45,共7页Environmental Ecology

基　　金：重点学科建设项目“麦地卡湿地生态站建设项目”阶段性成果,藏财预指(2019)01号;重点学科建设项目“西藏麦地卡湿地生态站建设项目(一期)”阶段性成果,藏财教指(2018)54号联合资助。

摘　　要：冰川棘豆(Oxytropis glacialis)是青藏高原特有种,具有很强的抗逆性,能在青藏高原多重胁迫条件下广泛分布,被认为是危害草原畜牧业生产最为严重的毒草。利用青藏高原不同生境条件下6个居群的17个冰川棘豆样本进行了转录组测序,结果显示:1)通过RNA-seq共得到956921788条Raw reads,质控后得到950938126条Clean reads,Q20(%)均大于98%,优化组装得到301738个unigenes,组装过滤后得到540308400条Clean reads,其中有338985738条比对到组装转录本上。2)unigenes的GO富集结果显示,富集程度较大的有细胞形状调节、高尔基囊泡介导的转运、色素的生物合成过程、脱落酸激活信号通路、肽基丝氨酸磷酸化与肽基丝氨酸修饰。KEGG富集表明,大部分unigenes被注释到光合作用、类胡萝卜素的生物合成、MAPK信号转导、植物激素信号转导以及植物与病原菌互作等相关通路。这些基因可能有利于冰川棘豆适应青藏高原低温、强辐射等极端环境,初步探究结果为进一步探讨其适应性分子机制和毒草防控提供了基础依据。Oxytropis glacialis is a unique species in the Qinghai-Tibet Plateau,which has strong resistance to stress and can be widely distributed under multiple stress conditions in the Qinghai-Tibet Plateau.It is considered to be the most serious poisonous grass endangering production of animal husbandry in the grassland.Transcriptome studies were conducted on 17 samples from Oxytropis glacialis of 6 populations in different habitats in the Qinghai-Tibet Plateau.The results showed that:1)956921788 raw reads were obtained by RNA-seq,950938126 clean reads were obtained by quality control,Q20(%)were more than 98%.301738 unigenes were obtained by optimized assembly,540308400 clean reads were obtained by filtration,of which 338985738 were compared to the assembled transcripts.2)Go enrichment results showed that,the more degree of enrichment were regulation of cell shape,intra-Golgi vesicle-mediated transport,pigment biosynthetic process,abscisic acid-activated signaling pathway,peptidyl-serine phosphorylation and peptidyl-serine modification.The KEGG enrichment showed that most of the unigene were annotated in photosynthesis,carotenoid biosynthesis,MAPK signaling pathway,plant hormone signal transduction and plant-pathogen interaction.These genes may be beneficial to acclimation of Oxytropis glacialis to the extreme environment of low temperature and strong radiation in the Tibetan Plateau,These preliminary results provide a basis for further study on the molecular mechanism of adaptation and control of poisonous weeds.

关 键 词：青藏高原 冰川棘豆 转录组测序 基因注释 适应性 

分 类 号：Q949.751.9[生物学—植物学]